Creating Yeast Populations with Bait and Prey Library
8:03
Sample Preparation for DEEPN Deep Sequencing
11:51
Results: DEEPN Screen Allows Wide-scale Protein Interaction Studies
13:00
Conclusion
Transcription
This method uses the principle of the yeast 2-hybrid system in both populations to identify protein-protein interactions that answer key questions about a variety of molecular and cellular processes. This technique leverages deep sequencing to see
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Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.