Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

8.4K Views

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12:22 min

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August 4th, 2018

DOI :

10.3791/57814-v

August 4th, 2018

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8,386 Views

Weiye Song*¹, Libo Zhou*¹, Ji Yi¹^,²

¹Department of Medicine, Boston University School of Medicine, ²Department of Biomedical Engineering, Boston University

Chapitres

0:04

Title

1:15

Oblique Scanning Laser Ophthalmoscopy (oSLO) System Setup

4:09

Optical Coherence Tomography System Setup

5:17

Data Acquisition

6:46

In Vivo Retinal Imaging

10:07

Results: Rat Retinal Image Acquired by Simultaneous oSLO and OCTA

11:35

Conclusion

Transcription

This method can help answer key questions in the field of ophthalmology and retinal imaging. Such as imaging and quantification of blood-retina barrier disruption and retinal capillary functions. The main advantage of this technique is that it can

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Résumé

Here, we present a protocol to get a large field of view (FOV) three-dimensional (3D) fluorescence and OCT retinal image by using a novel imaging multimodal platform. We will introduce the system setup, the method of alignment, and the operational protocols. In vivo imaging will be demonstrated, and representative results will be provided.

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Multimodal

Volumetric

Retinal Imaging

Oblique Scanning Laser Ophthalmoscopy oSLO

Optical Coherence Tomography OCT

Blood retina Barrier Disruption

Retinal Capillary Functions