Tissue Preparation, Delipidation, and Permeabilization
3:14
Whole Mount Immunostaining
4:42
Tissue Clearing
6:58
Results: 3D Imaging of Adipose Tissue
8:09
Conclusion
Transcription
This method can help answer key questions in the field of Adipose Tissue Biology, such as how different components of Adipose Tissue interact in an entire tissue. The main advantage of this technique is that it fully removes the excess lipids stor
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Due to the high lipid content, adipose tissue has been challenging to visualize using traditional histological methods. Adipo-Clear is a tissue clearing technique that allows robust labeling and high-resolution volumetric fluorescent imaging of adipose tissue. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for imaging.