Genomic DNA Isolation and Human T Cell Quantification
4:59
Results: Representative GVHD Disease Progression and Digital PCR Human T Cell Detection
5:37
Conclusion
Transcription
This protocol outlines how to induce xenograft-versus-host disease, or xenoGVHD, and how to blind and standardize clinical scoring to ensure consistent results. The xenoGVHD model provides an in vivo method for testing immunosuppressive therapies
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Here, we present a protocol to induce and score disease in a xenogeneic graft-versus-host disease (xenoGVHD) model. xenoGVHD provides an in vivo model to study immunosuppression of human T cells. Additionally, we describe how to detect human T cells in tissues with digital PCR as a tool to quantify immunosuppression.