Results: Analysis of Laser Capture Microdissected Tissue
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Conclusion
Transcription
The heterogeneity of skeletal structures is one obstacle in studying the gene expression in developing cartilage and bone. This protocol provides a method for precise isolation of adjacent but different tissues. We optimize the laser capture micro
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This protocol describes laser capture microdissection for the isolation of cartilage and bone from fresh frozen sections of the mouse embryo. Cartilage and bone can be rapidly visualized by cresyl violet staining and collected precisely to yield high quality RNA for transcriptomic analysis.