February 21st, 2020
•This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.
Vidéos Connexes
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
A Rapidly Incremented Tethered-Swimming Maximal Protocol for Cardiorespiratory Assessment of Swimmers
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays