Correlating Optical Density and Bacterial Concentration
2:14
VLP Attachment
3:29
Antibody Staining
5:19
Results: Quantifying VLP Binding
6:15
Conclusion
Transcription
Virus-bacterial interactions can be important for successful viral infection and stimulation of a host immune response. But currently, highly concentrated and purified stock of human norovirus cannot be produced in the lab. This technique can be u
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The goal of this protocol is to quantify binding of the eukaryotic pathogen human norovirus to bacteria. After performing an initial virus-bacterium attachment assay, flow cytometry is used to detect virally-bound bacteria within the population.