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A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

3.9K Views

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06:59 min

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May 21st, 2020

DOI :

10.3791/61350-v

May 21st, 2020

•

3,913 Views

Ashley L. Comer¹^,²^,³^,⁴, Balaji Sriram⁵, William W. Yen², Alberto Cruz-Martín¹^,²^,³^,⁴^,⁶

¹The Graduate Program for Neuroscience, Boston University, ²Department of Biology, Boston University, ³Neurophotonics Center, Boston University, ⁴Center for Systems Neuroscience, Boston University, ⁵Research and Early Development, Biogen, ⁶Department Pharmacology and Experimental Therapeutics, Boston University

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06:59

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

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19:04

In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos

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In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

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Method for Culture of Early Chick Embryos ex vivo (New Culture)

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Double Whole Mount in situ Hybridization of Early Chick Embryos

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Method for Whole Mount Antibody Staining in Chick

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Assay for Neural Induction in the Chick Embryo

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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

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