ALR/Antibody Complex Preparation and Invasion Assay Setup
3:25
Migration Assay Setup
4:18
Live-Cell Analysis Instrument Setting for Image Acquisition
5:14
Live-Cell Analysis Instrument Setting for Image Analysis
6:30
Results: Analysis of CD44 Expression in 3D Tumor Cell Invasion and Migration
8:03
Conclusion
Transcription
Diffuse midline glioma is a highly invasive brain tumor. This method helps to study in real time how DMG cells migrate and invade in a 3D context with specific insights on the potential role of a key adhesion molecule. By using a labeling reagent
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This study presents a protocol of live-3D-cell immunocytochemistry applied to a pediatric diffuse midline glioma cell line, useful to study in real-time the expression of proteins on the plasma membrane during dynamic processes like 3D cell invasion and migration.