Robotic Solid Phase Extraction for NMR Sample Preparation
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Results: Measurement of Arginine Methylome by NMR Spectroscopy
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Conclusion
Transcription
This protocol makes it possible to quantify levels and dynamics of arginine methylation. This modification plays important regulatory roles and could serve as a biomarker for disease. The advantage of this method is that it provides a simple workf
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The present protocol describes the preparation and quantitative measurement of free and protein-bound arginine and methyl-arginines by 1H-NMR spectroscopy.