Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
2:44
Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
3:56
Isothermal Titration Calorimetry
9:00
Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
10:48
Conclusion
Transcription
This method will allow the researcher to probe hard to study systems, like peptides and first row transition metal ions, both of which are generally challenging using more standard applications. The main advantage here is using orthogonal techniqu
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.