Results: Generating Human Microglia‐Like Cells and Synaptosomes for In Vitro Live‐Cell Phagocytosis Assay
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Conclusion
Transcription
iPS-derived microglia represent a biologically relevant source of human microglia for in vitro experimentation, and are an important tool to investigate microglia biology in health and disease. We present a simple microglia differentiation protoco
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This protocol describes the differentiation process of human induced pluripotent stem cells (iPSCs) into microglia-like cells for in vitro experimentation. We also include a detailed procedure for generating human synaptosomes from iPSC-derived lower motor neurons that can be used as a substrate for in vitro phagocytosis assays using live-cell imaging systems.