Electroporation of Mycobacteria
May 23rd, 2008
•Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for introducing DNA into mycobacterial cells.
Vidéos Connexes
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Electroporation of Craniofacial Mesenchyme
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
In Ovo Electroporation in Embryonic Chick Retina
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
In Vivo Microinjection and Electroporation of Mouse Testis
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