1. עכברוש המרה אנטומיה הקוטר הממוצע אורך צינור המרה והיה 1 מ&quot;מ ו 29 מ&quot;מ בהתאמה. המרחק הממוצע בין השוער לבין פטמית התריסריון היה 28 מ&quot;מ. ישנם שני צינורות הלבלב העיקרית אשר פתוח לתוך צינור המרה - צינור הקדמי פותח בצד שמאל את צינור האחורי נפתח בצד ימין. נוסף על אלה צינוריות גדולות, מספר צינורות ניקוז קטין כי כל שלושת החלקים של הלבלב לפתוח ישירות בצינור המרה. 6 2. טכניקה כל הניסויים בבעלי חיים בוצעו בהסכם עם המכון הלאומי לחקר הבריאות הנחיות אתיות אושרו על ידי ועדת המחקר של המוסד טיפוח ועדת בעלי חיים. זכר Sprague-Dawley חולדות בגילאי 8 שבועות שימשו הפגנה זו. חולדות התקבלו צ&#39;ארלס ריבר מעבדות בווילמינגטון, מסצ&#39;וסטס וכולם היו מעל 300 גרם. החולדות היתה גישה דיאטה מעבדה סטנדרטיים שוכנו יחד בתנאי מעבדה סטנדרטיים. החולדות היו בתחילה הרדים עם isofluorane ושמר תחת הרדמה במהלך הניתוח עם isofluorane דרך מסכה. חתך קו האמצע של 2 ס&quot;מ נעשתה החל xiphisternum. לאחר fascia נפתחה, האונות בכבד בוטלו cranially עם מפשק של סן, ואת התריסריון בוטלה caudally באמצעות Q-טיפים. בצינור המרה זוהה מבנה צינורי הזנת התריסריון. בצינור המרה הושם תחת מתח לחתוך חלקית. סטרילי 5 ס&quot;מ צינור פוליאתילן ארוך בקוטר פנימי של 0.6 מ&quot;מ ו קוטר חיצוני של 0.9 מ&quot;מ היה בשימוש. קצה אחד של הצינור הזה היה חום אטום, ואת הקצה השני היה משופעים בעדינות. סוף משופעים הועבר מכן לתוך צינור המרה. זה היה במקום מאובטח באמצעות 8-0 תפרים ניילון הן הפרוקסימלית ומ דיסטלי לפתיחת בצינור המרה. צינור הנותרים יחד עם סוף חתום הוצב חלל הצפק. האתר היה פעיל מושקים עם מלח רגיל. הבטן נסגר אז 2 שכבות, עם תפר Polyglactin 4-0. Linea alba נסגר עם התפרים רציפה ואת התפרים עורי קטע הונחו. דבק עור הוחל אז על העור. החולדות הורשו לשחזר בתנאי מעבדה רגילים, ניטור יומיומי משקל הגוף על מנת להבטיח הידרציה תקין. במשך המבצע היפוך cholestasis, החולדות היו מורדמים באמצעות isofluorane כפי שתואר לעיל. הבטן נפתחה על האתר החתך הקודם. הצינור היה מזוהה ונלקח מתוך חלל הצפק. Omental הידבקויות סביב הצינור משוחררים באמצעות לנתיחה בוטה וחד. אתר תריסריון זוהה לאחר מכן על מיקום נוח של הצינור duodenostomy. מחרוזת ארנק תפר, 2-3 מ&quot;מ קוטר, הוצב מכן באמצעות ניילון 8-0. התריסריון נפתח ואז בתוך תפר זה מחרוזת בארנק. סוף חתום של הצינור נחתך אז אורך מתאים. זרימה חופשית של מיצי מרה היתה מובטחת. סיום זה של הצינור הונח אז אל התריסריון ואת תפר מחרוזת הארנק היה התהדקו. הבטן היתה מושקים וסגר בשכבות. במחקר קודם, שבו choledochoduodenostomy בוצעה בטכניקה שונה, החולדות היו שרד במשך 12 שבועות וחולדות היו בריאים באותה תקופה. כמו כן, מחקר זה אישר את היפוך של שינויים ביוכימיים היסטולוגית לאחר choledochoduodenostomy בחולדות נתון חסימה של צינור המרה לתקופה של 2 שבועות. 7

<ol>
	<li>Gouma, D. J., Roughneen, P. T., Kumar, S., Moody, F. G., Rowlands, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+nutritional+status+associated+with+obstructive+jaundice+and+biliary+drainage+in+rats.">Changes in nutritional status associated with obstructive jaundice and biliary drainage in rats.</a> Am J Clin Nutr. 44, 362-369 (1986).</li><li>Ohtsuka, M., Miyazaki, M., Kondo, Y., Nakajima, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutrophil-mediated+sinusoidal+endothelial+cell+injury+after+extensive+hepatectomy+in+cholestatic+rats.">Neutrophil-mediated sinusoidal endothelial cell injury after extensive hepatectomy in cholestatic rats.</a> Hepatology. 25, 636-641 (1997).</li><li>Abe, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kupffer+cell-derived+interleukin+10+is+responsible+for+impaired+bacterial+clearance+in+bile+duct-ligated+mice.">Kupffer cell-derived interleukin 10 is responsible for impaired bacterial clearance in bile duct-ligated mice.</a> Hepatology. 40, 414-423 (2004).</li><li>Saiki, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preoperative+internal+biliary+drainage+is+superior+to+external+biliary+drainage+in+liver+regeneration+and+function+after+hepatectomy+in+obstructive+jaundiced+rats.">Preoperative internal biliary drainage is superior to external biliary drainage in liver regeneration and function after hepatectomy in obstructive jaundiced rats.</a> Ann Surg. 230, 655-662 (1999).</li><li>Subhas, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+methylprednisolone+on+warm+ischemia-reperfusion+injury+in+a+cholestatic+rat+liver.">Protective effect of methylprednisolone on warm ischemia-reperfusion injury in a cholestatic rat liver.</a> Am J Surg. 199, 377-380 (2010).</li><li>Kara, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+anatomical+study+on+the+rat+pancreas+and+its+ducts+with+emphasis+on+the+surgical+approach.">The anatomical study on the rat pancreas and its ducts with emphasis on the surgical approach.</a> Ann Anat. 187, 105-112 (2005).</li><li>Ryan, C. J., Than, T., Blumgart, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Choledochoduodenostomy+in+the+rat+with+obstructive+jaundice.">Choledochoduodenostomy in the rat with obstructive jaundice.</a> J Surg Res. 23, 321-331 (1977).</li></ol>

אין ניגודי אינטרסים הכריז.

Cholestasis מוביל מספר עצום של שינויים במערכת העיכול. מחקרים שנעשו מחפש את השינויים במערכת החיסונית ואת מצב תזונתי. Cholestasis 1-3 יכולים להשפיע על תוצאות של ניתוחים בכבד. מחקרים בוצעו כדי לשפר את התוצאות של ניתוחי הכבד בנוכחות cholestasis. 4-5 עם שיפור הרפואה והמחקר, מחקרי...

creation of reversible cholestatic rat model

Cholestasis הוא מצב קליני נתקל בדרך כלל על ידי שני מנתחים גסטרואנטרולוגים. Cholestasis יכול לגרום לשינויים פיזיולוגיים שונים משפיעים על מצב תזונתי ותוצאות הניתוח. חקר השינויים המתרחשים pathophysiological הכבד ואיברים אחרים הוא בעל חשיבות. מחקרים שונים שנעשו במודלים של חולדה cholestatic. השתמשנו במודל הפיך עכברוש cholestatic במחקר האחרונות שלנו מסתכל התפקיד של methylprednisolone לפציעתם reperfusion איסכמיה. טכניקות שונות ליצירת מודל cholestatic הפיך תוארו. יצירה של מודל cholestatic הפיך עכברוש יכול להיות מאתגר לאור גודל קטן האנטומיה hepatopancreatobiliary ייחודי בחולדות. מאמר זה מדגים וידאו ויצירת מודל cholestatic הפיך. מודל זה יכול לשמש במחקרים שונים, כמו להסתכל על שינויים תזונתיים, פיזיולוגיים, שינויים פתולוגיים היסטולוגית ו החיסונית במערכת העיכול. מודל זה יכול לשמש גם כדי לראות את ההשפעות של cholestasis ו התערבויות טיפוליות שונות על ניתוחים הכבד הגדולות.

Watch this Scientific Journal Video about Creation of Reversible Cholestatic Rat Model at JoVE.com

Creation of Reversible Cholestatic Rat Model

Cholestasis הוא מצב קליני נתקל בדרך כלל על ידי שני מנתחים גסטרואנטרולוגים. יצירה של מודל cholestatic הפיך עכברוש יכול להיות מאתגר לאור גודל קטן האנטומיה hepatopancreatobiliary ייחודי בחולדות. מאמר זה מדגים וידאו ויצירת מודל cholestatic הפיך.

יצירתו של מודל הפיך עכברוש Cholestatic

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and ...

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14.9K Views.  Providence Hospital and Medical Centers. Cholestasis הוא מצב קליני נתקל בדרך כלל על ידי שני מנתחים גסטרואנטרולוגים. יצירה של מודל cholestatic הפיך עכברוש יכול להיות מאתגר לאור גודל קטן האנטומיה hepatopancreatobiliary ייחודי בחולדות. מאמר זה מדגים וידאו ויצירת מודל cholestatic הפיך.

Video: Creation of Reversible Cholestatic Rat Model

A Model of Chronic Nutrient Infusion in the Rat

Chronic exposure to excessive levels of nutrients is postulated to affect the function of several organs and tissues and to contribute to the development of the many complications associated with obesity and the metabolic syndrome, including type 2 diabetes. To study the mechanisms by which excessive levels of glucose and fatty acids affect the pancreatic beta-cell and the secretion of insulin, we have established a chronic nutrient infusion model in the rat. The procedure consists of catheterizing the right jugular vein and left carotid artery under general anesthesia; allowing a 7-day recuperation period; connecting the catheters to the pumps using a swivel and counterweight system that enables the animal to move freely in the cage; and infusing glucose and/or Intralipid (a soybean oil emulsion which generates a mixture of approximately 80% unsaturated/20% saturated fatty acids when infused with heparin) for 72 hr. This model offers several advantages, including the possibility to finely modulate the target levels of circulating glucose and fatty acids; the option to co-infuse pharmacological compounds; and the relatively short time frame as opposed to dietary models. It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness of drugs in this context.

A protocol for chronic infusions of glucose and Intralipid in rats is described. This model can be used to study the impact of nutrient excess on organ function and physiological parameters.

דגם של עירוי מזין כרוני בחולדה

Chronic exposure to excessive levels of nutrients is postulated to  affect the function of several organs and tissues and to contribute to the  ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart failure. Here, we describe a detailed surgical procedure for creating pressure overload and cardiac hypertrophy in rats by constriction of the ascending aorta using a small metallic clip. After anesthesia, the trachea is intubated by inserting a cannula through a half way incision made between two cartilage rings of trachea. Then a skin incision is made at the level of the second intercostal space on the left chest wall and muscle layers are cleared to locate the ascending portion of aorta. The ascending aorta is constricted to 50&#8211;60% of its original diameter by application of a small sized titanium clip. Following aortic constriction, the second and third ribs are approximated with prolene sutures. The tracheal cannula is removed once spontaneous breathing was re-established. The animal is allowed to recover on the heating pad by gradually lowering anesthesia. The intensity of pressure overload created by constriction of the ascending aorta is determined by recording the pressure gradient using trans-thoracic two dimensional Doppler-echocardiography. Overall this protocol is useful to study the remodeling events and contractile properties of the heart during the gradual onset and progression from compensated cardiac hypertrophy to heart failure stage.

We describe a stepwise procedure for creating pressure overload and left ventricular hypertrophy in Wistar rats by constriction of the ascending aorta using a small metallic clip. This model is extensively used for studying remodeling changes during cardiac hypertrophy and for identifying and evaluating strategies for regression of such changes.

עולה אבי העורקים תעוקה בחולדות ליצירת עומס יתר לחץ לב היפרטרופיה הדגם

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy

Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history have&#160;remained poorly understood. Recently, we developed a reliable, reproducible animal model of PION and tested the treatment effect of some neurotrophic factors in this model1. The purpose of this video is to demonstrate our photochemically induced model of posterior ischemic optic neuropathy, and to evaluate its effects with retrograde labeling of retinal ganglion cells. Following surgical exposure of the posterior optic nerve, a photosensitizing dye, erythrosin B, is intravenously injected and a laser beam is focused onto the optic nerve surface. Photochemical interaction of erythrosin B and the laser during irradiation damages the vascular endothelium, prompting microvascular occlusion mediated by platelet thrombosis and edematous compression. The resulting ischemic injury yields a gradual but pronounced retinal ganglion cell dieback, owing to a loss of axonal input - a remote, injury-induced and clinically relevant outcome. Thus, this model provides a novel platform to study the pathophysiologic course of PION,&#160;and can be further optimized for testing therapeutic approaches for optic neuropathies as well as other CNS ischemic diseases.

The goal of this protocol is to photochemically induce ischemic injury to the posterior optic nerve in rat. This model is critical to studies of the pathophysiology of posterior ischemic optic neuropathy, and therapeutic approaches for this and other optic neuropathies, as well as of other CNS ischemic diseases.

מודל עכברוש של אחורי איסכמי אופטי נוירופתיה המושרית photochemically

Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history ...

A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction

Obstruction of the kidney may affect native or transplanted kidneys and results in kidney injury and scarring. Presented here is a model of obstructive nephropathy induced by unilateral ureteric obstruction (UUO), which can either be irreversible (UUO) or reversible (R-UUO). In the irreversible UUO model, the ureter may be obstructed for variable periods of time in order to induce increasingly severe renal inflammation and interstitial fibrotic scarring. In the reversible R-UUO model the ureter is obstructed to induce hydronephrosis, tubular dilation and inflammation. After a suitable period of time the ureteric obstruction is then surgically reversed by anastomosis of the severed previously obstructed ureter to the bladder in order to allow complete decompression of the kidney and restoration of urinary flow to the bladder. The irreversible UUO model has been used to investigate various aspects of renal inflammation and scarring including the pathogenesis of disease and the testing of potential anti-inflammatory or anti-fibrotic therapies. The more challenging model of R-UUO has been used by some investigators and does offer significant research potential as it allows the study of inflammatory and immune processes and tissue remodeling in an injured and scarred kidney following the removal of the injurious stimulus. As a result, the R-UUO model offers investigators the opportunity to explore the resolution of kidney inflammation together with key aspects of tissue repair. These experimental models are of relevance to human disease as patients often present with obstruction of the renal tract that requires decompression and are commonly left with significant residual kidney impairment that has no current treatment options and may lead to eventual end stage kidney failure.

The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.

דגם Murine של חסימת Ureteric חד-צדדית בלתי הפיכה והפיכה

Obstruction of the kidney may affect native or transplanted kidneys and results in kidney injury and scarring. Presented here is a model of obstructive ...

Demonstration of the Rat Ischemic Skin Wound Model

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and unrelieved pressure due to immobility. Animal models have been developed that attempt to mimic these conditions for the purpose of furthering our understanding of the complexity of chronic wounds. The model described herein is a rat ischemic skin flap model that permits a prolonged reduction of blood flow resulting in wounds that become ischemic and resemble a chronic wound phenotype (reduced vascularization, increased inflammation and delayed wound closure). It consists of a bipedicled dorsal flap with 2 ischemic wounds placed centrally and 2 non-ischemic wounds lateral to the flap as controls. A novel addition to this ischemic skin flap model is the placement of a silicone sheet beneath the flap that functions as a barrier and a splint to prevent revascularization and reduce contraction as the wounds heal. Despite the debate of using rats for wound healing studies due to their quite distinct anatomic and physiologic differences compared to humans (i.e., the presence of a panniculus carnosus muscle, short life-span, increased number of hair follicles, and their ability to heal infected wounds) the modifications employed in this model make it a valuable alternative to previously developed ischemic skin flap models.

The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.

הפגנה של דגם פצע עור העכברוש איסכמי

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and ...

Creation of Abdominal Adhesions in Mice

Abdominal adhesions consist of fibrotic tissue that forms in the peritoneal space in response to an inflammatory insult, typically surgery or intraabdominal infection. The precise mechanisms underlying adhesion formation are poorly understood. Many compounds and physical barriers have been tested for their ability to prevent adhesions after surgery with varying levels of success. The mouse and rat are important models for the study of abdominal adhesions. Several different techniques for the creation of adhesions in the mouse and rat exist in the literature. Here we describe a protocol utilizing abrasion of the cecum with sandpaper and sutures placed in the right abdominal sidewall. The mouse is anesthetized and the abdomen is prepped. A midline laparotomy is created and the cecum is identified. Sandpaper is used to gently abrade the surface of the cecum. Next, several figure-of-eight sutures are placed into the peritoneum of the right abdominal sidewall. The abdominal cavity is irrigated, a small amount of starch is applied, and the incision is closed. We have found that this technique produces the most consistent adhesions with the lowest mortality rate.

Abdominal adhesions that form after surgery are a major cause of pain, infertility, and hospitalization and reoperation for small bowel obstruction. Our surgical procedure for creating abdominal adhesions in mice is a reliable tool to study the mechanisms underlying the formation of adhesions.

יצירת הידבקויות בטן עכברים

Abdominal adhesions consist of fibrotic tissue that forms in the peritoneal space in response to an inflammatory insult, typically surgery or ...

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients with impeded blood flow or with large wounds might be prolonged. Cell-based therapies have appeared as a new technique for the treatment of diabetic ulcers, and cell-sheet engineering has improved the efficacy of cell transplantation. A number of reports have suggested that adipose-derived stem cells (ASCs), a type of mesenchymal stromal cell (MSC), exhibit therapeutic potential due to their relative abundance in adipose tissue and their accessibility for collection when compared to MSCs from other tissues. Therefore, ASCs appear to be a good source of stem cells for therapeutic use. In this study, ASC sheets from the epididymal adipose fat of normal Lewis rats were successfully created using temperature-responsive culture dishes and normal culture medium containing ascorbic acid. The ASC sheets were transplanted into Zucker diabetic fatty (ZDF) rats, a rat model of type 2 diabetes and obesity, that exhibit diminished wound healing. A wound was created on the posterior cranial surface, ASC sheets were transplanted into the wound, and a bilayer artificial skin was used to cover the sheets. ZDF rats that received ASC sheets had better wound healing than ZDF rats without the transplantation of ASC sheets. This approach was limited because ASC sheets are sensitive to dry conditions, requiring the maintenance of a moist wound environment. Therefore, artificial skin was used to cover the ASC sheet to prevent drying. The allogenic transplantation of ASC sheets in combination with artificial skin might also be applicable to other intractable ulcers or burns, such as those observed with peripheral arterial disease and collagen disease, and might be administered to patients who are undernourished or are using steroids. Thus, this treatment might be the first step towards improving the therapeutic options for diabetic wound healing.

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.

יצירת והשתלות של גיליון נגזר שומן בתאי גזע (ASC) במודל של ריפוי פצע סוכרתי

Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic aneurysms (AAAs). This article introduces and describes a standard protocol used to induce AAAs through adventitial VV hypoperfusion created with a combination of polyurethane catheter insertion into the aortic lumen and suture ligation of the infrarenal abdominal aorta.
The protocol involves the use of male rats weighing 300-400 g, which are provided food and water ad libitum. After laparotomy with a ventral midline abdominal incision, exfoliation of the aorta is performed, which blocks blood flow from the perivascular tissue. Aortotomy involving a small incision adjacent to the renal artery branches is performed, and a polyurethane catheter is inserted using an 18-gauge indwelling needle. After repairing the incision, tight ligation of the aorta over the catheter blocks VV blood flow from the proximal direction through the aortic wall without disturbing the aortic blood flow. This technique can induce an AAA with progressive aortic dilatation.
The greatest benefit of this model is that VV hypoperfusion causes tissue hypoxia and the development of an infrarenal AAA, which has morphological and pathological characteristics similar to those of a human AAA.

Polyurethane catheter insertion into the aortic lumen and suture ligation of the aorta induce chronic hypoxia due to hypoperfusion of the adventitial vasa vasorum. This article describes a novel animal model of abdominal aortic aneurysm (AAA) with characteristics similar to those of AAA in humans.

יצירת מודל מכרסם של מפרצת אבי העורקים בבטן על ידי חסימת ואסה Adventitial וזורום זלוף

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

Contractility איזומטרי מדידה של עורק מצע המעי העליון העכבר באמצעות חוט Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Creation of Colonic Anastomosis in Mice

Intestinal anastomoses are commonly performed in both elective and emergent operations. Even so, anastomotic leaks are a highly feared complications of colonic surgeries and can occur in up to 26% of surgical anastomoses, with mortality being up to 39% for patients with such a leak. Currently, there remains a paucity of data detailing the cellular mechanisms of anastomotic healing. Devising preventative strategies and treatment modalities for anastomotic leak could be greatly potentiated by a better understanding of appropriate anastomotic healing.
A murine model is ideal as previous studies have shown that the murine anastomosis is the most clinically similar to the human case as compared with other animal models. We offer an easily reproducible murine model of colonic anastomosis in mice that will allow for further illustration of anastomotic healing.

Anastomotic leak or breakdown after surgery is a major cause of postoperative morbidity and mortality. Our surgery for creating a colonic anastomosis is a reliable and reproducible method for studying the healing mechanisms of anastomoses.

יצירה של השקה שטיפת המעי הגס בעכברים

Intestinal anastomoses are commonly performed in both elective and emergent operations. Even so, anastomotic leaks are a highly feared complications of ...