1. 쥐 담즙의 해부학 평균 담즙 덕트 직경 및 길이는 각각 1mm와 29mm였다. pylorus과 십이지장 유두 사이의 평균 거리는 28mm했습니다. 담즙 덕트에 열 두 가지 주요 췌장 덕트가있다 - 앞쪽에 덕트는 왼쪽에 열리고 후부 덕트의 오른쪽에 열립니다. 이러한 주요 덕트 이외에 췌장의 세 부분을 물기 사소한 관의 숫자가 담즙 덕트에 직접 열려 6. 2. 기술 모든 동물 실험 윤리 연구를위한 건강 지침의 국립 연구소와 계약에 수행되었으며 기관의 연구위원회 및 동물 관리위원회에 의해 승인되었습니다. 팔주 세 남자 스프 라그 - 돌리 쥐이 실험을 위해 사용되었습니다. 쥐 찰스 강 윌밍턴에 연구소, MA 및 모든 300g 이상했다 얻은했다. 쥐 표준 실험실 다이어트에 대한 액세스를했고 표준 실험실 조건에서 함께 보관되어 있었다. 쥐 처음 isofluorane와 anesthetized되었고 마스크를 통해 isofluorane와 수술 중 마취 유지. 2cm의 정중선 절개는 흉부의 검상 부부부터 만들어졌다. 일단 근막은 간 엽 (叶)가 센의 견인기와 cranially 철회했고 오픈, 그리고 십이지장은 caudally Q - 팁을 사용하여 철회했다. 담즙 덕트는 십이지장을 입력 관형 구조로 확인되었다. 담즙 관은 긴장에 따라 배치하고 부분적으로 오픈 컷했습니다. 0.6 mm와 0.9 mm의 외부 직경의 내경과 멸균 5cm 길이 폴리에틸렌 관을 사용했습니다. 이 튜브의 한쪽 끝을 열이 봉인되었고 다른 끝을 부드럽게 beveled되었습니다. beveled 끝을 다음 담즙 덕트에 통과되었다. 이것은 근위 및 담즙 덕트의 개방에 말초 모두 8-0 나일론 봉합을 사용하여 장소에 확보했다. 밀봉된 엔드와 함께 남아있는 튜브는 복막 캐비티에 배치되었다. 효과적인 사이트는 정상적인 생리와 irrigated했다. 복부는 다음 4-0 Polyglactin의 봉합과 함께 2 레이어에 폐쇄되었습니다. linea 알바는 연속 봉합과 함께 폐쇄되었으며 중단 피부 봉합이 배치되었습니다. 피부 접착제 그런 다음 피부에 적용되었습니다. 쥐가은 적절한 보습을 위해 매일 체중 모니터링 표준 실험실 조건에서 복구할 수있었습니다. cholestasis 반전 작업, 쥐들이 앞에서 설명한 것처럼 isofluorane를 사용 anaesthetized되었습니다. 복부는 이전 절개 사이트를 통해 오픈했습니다. 튜브는 식별 및 복막 캐비티에서 제거되었습니다. 튜브 주위 Omental adhesions는 무딘과 날카로운 해부를 사용하여 해제됩니다. 십이지장에있는 사이트는 다음 duodenostomy 튜브의 편안한 위치에 대한 확인되었다. 지갑 문자열 봉합, 직경 2~3mm은 다음 8-0 나일론을 사용하여 배치했다. 십이지장은 다음이 지갑 문자열 봉합 안에 문을 열었습니다. 튜브의 봉인 끝에 그런 다음 적절한 길이로 절단했다. 담즙의 흐름을 무료로 보장했다. 튜브의 끝이 다음 십이지장에 배치되었고 지갑 문자열 봉합이 강화되었습니다. 복부는 레이어에서 irrigated 및 폐쇄되었습니다. 이전 연구에서 궁극적 choledochoduodenostomy가 다른 기술을 사용하여 수행되었다, 쥐 12 주 동안 살아와 쥐 그 기간 동안 건강하게 하였다. 또한이 연구는 2 주 기간 동안의 담즙 덕트의 방해를 받게 쥐에 choledochoduodenostomy 이후 생화학 및 histological 변경 반전을 확인 7.

<ol>
	<li>Gouma, D. J., Roughneen, P. T., Kumar, S., Moody, F. G., Rowlands, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+nutritional+status+associated+with+obstructive+jaundice+and+biliary+drainage+in+rats.">Changes in nutritional status associated with obstructive jaundice and biliary drainage in rats.</a> Am J Clin Nutr. 44, 362-369 (1986).</li><li>Ohtsuka, M., Miyazaki, M., Kondo, Y., Nakajima, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutrophil-mediated+sinusoidal+endothelial+cell+injury+after+extensive+hepatectomy+in+cholestatic+rats.">Neutrophil-mediated sinusoidal endothelial cell injury after extensive hepatectomy in cholestatic rats.</a> Hepatology. 25, 636-641 (1997).</li><li>Abe, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kupffer+cell-derived+interleukin+10+is+responsible+for+impaired+bacterial+clearance+in+bile+duct-ligated+mice.">Kupffer cell-derived interleukin 10 is responsible for impaired bacterial clearance in bile duct-ligated mice.</a> Hepatology. 40, 414-423 (2004).</li><li>Saiki, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preoperative+internal+biliary+drainage+is+superior+to+external+biliary+drainage+in+liver+regeneration+and+function+after+hepatectomy+in+obstructive+jaundiced+rats.">Preoperative internal biliary drainage is superior to external biliary drainage in liver regeneration and function after hepatectomy in obstructive jaundiced rats.</a> Ann Surg. 230, 655-662 (1999).</li><li>Subhas, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+methylprednisolone+on+warm+ischemia-reperfusion+injury+in+a+cholestatic+rat+liver.">Protective effect of methylprednisolone on warm ischemia-reperfusion injury in a cholestatic rat liver.</a> Am J Surg. 199, 377-380 (2010).</li><li>Kara, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+anatomical+study+on+the+rat+pancreas+and+its+ducts+with+emphasis+on+the+surgical+approach.">The anatomical study on the rat pancreas and its ducts with emphasis on the surgical approach.</a> Ann Anat. 187, 105-112 (2005).</li><li>Ryan, C. J., Than, T., Blumgart, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Choledochoduodenostomy+in+the+rat+with+obstructive+jaundice.">Choledochoduodenostomy in the rat with obstructive jaundice.</a> J Surg Res. 23, 321-331 (1977).</li></ol>

관심 없음 충돌 선언하지 않습니다.

Cholestasis은 위장 시스템의 변화 수많은로 연결됩니다. 연구는 면역 시스템과 영양 상태의 변화를 찾고 완료되었습니다. 1-3 Cholestasis는 간장 수술 결과에 영향을 줄 수 있습니다. 연구가 cholestasis의 존재에 간장 수술의 결과를 개선하기 위해 수행되었습니다. 의학 및 연구 개선으로 4-5, 추가 연구 cholestasis에서 수행됩니다. 따라서 그것은 좋은 cholestatic 모델을 가지고 필요합니다. 쥐...

creation of reversible cholestatic rat model

Cholestasis은 일반적으로 외과의와 소화기 전문 내과 모두에 의해 발생 임상 조건입니다. Cholestasis 다양한 생리 변화를 원인과 영양 상태 및 수술 결과에 영향을 미칠 수 있습니다. 간 및 다른 장기에서 발생 pathophysiological 변화 연구 중요하다. 다양한 연구 cholestatic 쥐 모델에서 수행되었습니다. 우리는 국소 빈혈 reperfusion의 부상에 methylprednisolone의 역할을 보는 우리의 최근 연구에서 가역 cholestatic 랫 모델을 사용합니다. 가역 cholestatic 모델의 창출을위한 다양한 기법을 설명하고 있습니다. 가역 cholestatic 랫 모델의 생성 쥐의 작은 크기와 독특한 hepatopancreatobiliary 해부학보기에 도전하실 수 있습니다. 이 동영상이 문서는 가역 cholestatic 모델의 생성을 보여줍니다. 이 모델은 같은 위장관의 영양, 생리, 병리, histological 및 면역 변화의 변화를보고 등 다양한 연구에 사용할 수 있습니다. 이 모델은 또한 cholestasis 주요 간장 수술에 대한 다양한 치료 개입의 효과를 볼 수 있습니다.

Watch this Scientific Journal Video about Creation of Reversible Cholestatic Rat Model at JoVE.com

Creation of Reversible Cholestatic Rat Model

Cholestasis은 일반적으로 외과의와 소화기 전문 내과 모두에 의해 발생 임상 조건입니다. 가역 cholestatic 랫 모델의 생성 쥐의 작은 크기와 독특한 hepatopancreatobiliary 해부학보기에 도전하실 수 있습니다. 이 동영상이 문서는 가역 cholestatic 모델의 생성을 보여줍니다.

가역 Cholestatic 쥐 모델의 생성

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and ...

creation-of-reversible-cholestatic-rat-model

Research

JoVE Journal

Medicine

14.9K 조회수.  Providence Hospital and Medical Centers. Cholestasis은 일반적으로 외과의와 소화기 전문 내과 모두에 의해 발생 임상 조건입니다. 가역 cholestatic 랫 모델의 생성 쥐의 작은 크기와 독특한 hepatopancreatobiliary 해부학보기에 도전하실 수 있습니다. 이 동영상이 문서는 가역 cholestatic 모델의 생성을 보여줍니다.

비디오: Creation of Reversible Cholestatic Rat Model

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart failure. Here, we describe a detailed surgical procedure for creating pressure overload and cardiac hypertrophy in rats by constriction of the ascending aorta using a small metallic clip. After anesthesia, the trachea is intubated by inserting a cannula through a half way incision made between two cartilage rings of trachea. Then a skin incision is made at the level of the second intercostal space on the left chest wall and muscle layers are cleared to locate the ascending portion of aorta. The ascending aorta is constricted to 50&#8211;60% of its original diameter by application of a small sized titanium clip. Following aortic constriction, the second and third ribs are approximated with prolene sutures. The tracheal cannula is removed once spontaneous breathing was re-established. The animal is allowed to recover on the heating pad by gradually lowering anesthesia. The intensity of pressure overload created by constriction of the ascending aorta is determined by recording the pressure gradient using trans-thoracic two dimensional Doppler-echocardiography. Overall this protocol is useful to study the remodeling events and contractile properties of the heart during the gradual onset and progression from compensated cardiac hypertrophy to heart failure stage.

We describe a stepwise procedure for creating pressure overload and left ventricular hypertrophy in Wistar rats by constriction of the ascending aorta using a small metallic clip. This model is extensively used for studying remodeling changes during cardiac hypertrophy and for identifying and evaluating strategies for regression of such changes.

압력 과부하 심장 비대 모델의 창조를위한 쥐의 대동맥 수축 오름차순

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

연구

의학

A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction

Obstruction of the kidney may affect native or transplanted kidneys and results in kidney injury and scarring. Presented here is a model of obstructive nephropathy induced by unilateral ureteric obstruction (UUO), which can either be irreversible (UUO) or reversible (R-UUO). In the irreversible UUO model, the ureter may be obstructed for variable periods of time in order to induce increasingly severe renal inflammation and interstitial fibrotic scarring. In the reversible R-UUO model the ureter is obstructed to induce hydronephrosis, tubular dilation and inflammation. After a suitable period of time the ureteric obstruction is then surgically reversed by anastomosis of the severed previously obstructed ureter to the bladder in order to allow complete decompression of the kidney and restoration of urinary flow to the bladder. The irreversible UUO model has been used to investigate various aspects of renal inflammation and scarring including the pathogenesis of disease and the testing of potential anti-inflammatory or anti-fibrotic therapies. The more challenging model of R-UUO has been used by some investigators and does offer significant research potential as it allows the study of inflammatory and immune processes and tissue remodeling in an injured and scarred kidney following the removal of the injurious stimulus. As a result, the R-UUO model offers investigators the opportunity to explore the resolution of kidney inflammation together with key aspects of tissue repair. These experimental models are of relevance to human disease as patients often present with obstruction of the renal tract that requires decompression and are commonly left with significant residual kidney impairment that has no current treatment options and may lead to eventual end stage kidney failure.

The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.

돌이킬 수 및 가역 일방적 Ureteric 폐색의 쥐 모델

Obstruction of the kidney may affect native or transplanted kidneys and results in kidney injury and scarring. Presented here is a model of obstructive ...

Creation of Abdominal Adhesions in Mice

Abdominal adhesions consist of fibrotic tissue that forms in the peritoneal space in response to an inflammatory insult, typically surgery or intraabdominal infection. The precise mechanisms underlying adhesion formation are poorly understood. Many compounds and physical barriers have been tested for their ability to prevent adhesions after surgery with varying levels of success. The mouse and rat are important models for the study of abdominal adhesions. Several different techniques for the creation of adhesions in the mouse and rat exist in the literature. Here we describe a protocol utilizing abrasion of the cecum with sandpaper and sutures placed in the right abdominal sidewall. The mouse is anesthetized and the abdomen is prepped. A midline laparotomy is created and the cecum is identified. Sandpaper is used to gently abrade the surface of the cecum. Next, several figure-of-eight sutures are placed into the peritoneum of the right abdominal sidewall. The abdominal cavity is irrigated, a small amount of starch is applied, and the incision is closed. We have found that this technique produces the most consistent adhesions with the lowest mortality rate.

Abdominal adhesions that form after surgery are a major cause of pain, infertility, and hospitalization and reoperation for small bowel obstruction. Our surgical procedure for creating abdominal adhesions in mice is a reliable tool to study the mechanisms underlying the formation of adhesions.

마우스의 복부 유착의 창조

Abdominal adhesions consist of fibrotic tissue that forms in the peritoneal space in response to an inflammatory insult, typically surgery or ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic aneurysms (AAAs). This article introduces and describes a standard protocol used to induce AAAs through adventitial VV hypoperfusion created with a combination of polyurethane catheter insertion into the aortic lumen and suture ligation of the infrarenal abdominal aorta.
The protocol involves the use of male rats weighing 300-400 g, which are provided food and water ad libitum. After laparotomy with a ventral midline abdominal incision, exfoliation of the aorta is performed, which blocks blood flow from the perivascular tissue. Aortotomy involving a small incision adjacent to the renal artery branches is performed, and a polyurethane catheter is inserted using an 18-gauge indwelling needle. After repairing the incision, tight ligation of the aorta over the catheter blocks VV blood flow from the proximal direction through the aortic wall without disturbing the aortic blood flow. This technique can induce an AAA with progressive aortic dilatation.
The greatest benefit of this model is that VV hypoperfusion causes tissue hypoxia and the development of an infrarenal AAA, which has morphological and pathological characteristics similar to those of a human AAA.

Polyurethane catheter insertion into the aortic lumen and suture ligation of the aorta induce chronic hypoxia due to hypoperfusion of the adventitial vasa vasorum. This article describes a novel animal model of abdominal aortic aneurysm (AAA) with characteristics similar to those of AAA in humans.

Adventitial 바사 Vasorum 관류를 차단 하 여 복 부 대동맥 동맥 류의 설치류 모델의 생성

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

Myography 와이어를 사용 하 여 마우스 Mesenteric 동맥의 isometric 수축 측정

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Creation of Colonic Anastomosis in Mice

Intestinal anastomoses are commonly performed in both elective and emergent operations. Even so, anastomotic leaks are a highly feared complications of colonic surgeries and can occur in up to 26% of surgical anastomoses, with mortality being up to 39% for patients with such a leak. Currently, there remains a paucity of data detailing the cellular mechanisms of anastomotic healing. Devising preventative strategies and treatment modalities for anastomotic leak could be greatly potentiated by a better understanding of appropriate anastomotic healing.
A murine model is ideal as previous studies have shown that the murine anastomosis is the most clinically similar to the human case as compared with other animal models. We offer an easily reproducible murine model of colonic anastomosis in mice that will allow for further illustration of anastomotic healing.

Anastomotic leak or breakdown after surgery is a major cause of postoperative morbidity and mortality. Our surgery for creating a colonic anastomosis is a reliable and reproducible method for studying the healing mechanisms of anastomoses.

쥐에 있는 저 승 문 합의 창조

Intestinal anastomoses are commonly performed in both elective and emergent operations. Even so, anastomotic leaks are a highly feared complications of ...

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing stable IOP elevation in mice that mimics the post-operative complication of using silicone oil (SO) as a tamponade agent in human vitreoretinal surgery. In this protocol, SO is injected into the anterior chamber of the mouse eye to block the pupil and prevent inflow of aqueous humor. The posterior chamber accumulates aqueous humor and this in turn increases the IOP of the posterior segment. A single SO injection produces reliable, sufficient, and stable IOP elevation, which induces significant glaucomatous neurodegeneration. This model is a true replicate of secondary glaucoma in the eye clinic. To further mimic the clinical setting, SO can be removed from the anterior chamber to reopen the drainage pathway and allow inflow of aqueous humor, which is drained through the trabecular meshwork (TM) at the angle of the anterior chamber. Because IOP quickly returns to normal, the model can be used to test the effect of lowering IOP on glaucomatous retinal ganglion cells. This method is straightforward, does not require special equipment or repeat procedures, closely simulates clinical situations, and may be applicable to diverse animal species. However, minor modifications may be required.

Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to normal.

Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing ...

Acupuncture in a Rat Model of Asthma

A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes methods for the fabrication of the high platform, establishes a rat model of asthma and measures changes in respiratory function using a noninvasive and real-time whole-body plethysmography (WBP) system.

천식의 쥐 모형에 있는 침술

A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes ...

Retinal Pathophysiological Evaluation in a Rat Model

A posterior segment eye disease like diabetic retinopathy alters the physiology of the retina. Diabetic retinopathy is characterized by a retinal detachment, breakdown of the blood-retinal barrier (BRB), and retinal angiogenesis. An in vivo rat model is a valuable experimental tool to examine the changes in the structure and function of the retina. We propose three different experimental techniques in the rat model to identify morphological changes of retinal cells, retinal vasculature, and compromised BRB. Retinal histology is used to study the morphology of various retinal cells. Also, quantitative measurement is performed by retinal cell count and thickness measurement of different retinal layers. A BRB breakdown assay is used to determine the leakage of extraocular proteins from the plasma to vitreous tissue due to the breakdown of BRB. Fluorescence angiography is used to study angiogenesis and leakage of blood vessels by visualizing retinal vasculature using FITC-dextran dye.

Diabetic retinopathy is one of the leading causes of blindness. Histology, blood-retinal barrier breakdown assay, and fluorescence angiography are valuable techniques to understand the pathophysiology of the retina, which could further enhance the efficient drug screening against diabetic retinopathy.

쥐 모델에서의 망막 병리생리학적 평가

A posterior segment eye disease like diabetic retinopathy alters the physiology of the retina. Diabetic retinopathy is characterized by a retinal ...

Development of a Uterosacral Ligament Suspension Rat Model

Pelvic organ prolapse (POP) is a common pelvic floor disorder (PFD) with the potential to significantly impact a woman's quality of life. Approximately 10%-20% of women undergo pelvic floor repair surgery to treat prolapse in the United States. PFD cases result in an overall $26.3 billion annual cost in the United States alone. This multifactorial condition has a negative impact on the quality of life and yet the treatment options have only dwindled in the recent past. One common surgical option is uterosacral ligament suspension (USLS), which is typically performed by affixing the vaginal vault to the uterosacral ligament in the pelvis. This repair has a lower incidence of complications compared to those with mesh augmentation, but is notable for a relatively high failure rate of up to 40%. Considering the lack of standard animal models to study pelvic floor dysfunction, there is an urgent clinical need for innovation in this field with a focus on developing cost-effective and accessible animal models. In this manuscript, we describe a rat model of USLS involving a complete hysterectomy followed by fixation of the remaining vaginal vault to the uterosacral ligament. The goal of this model is to mimic the procedure performed on women to be able to use the model to then investigate reparative strategies that improve the mechanical integrity of the ligament attachment. Importantly, we also describe the development of an in situ tensile testing procedure to characterize interface integrity at chosen time points following surgical intervention. Overall, this model will be a useful tool for future studies that investigate treatment options for POP repair via USLS.

Pelvic organ prolapse affects millions of women worldwide and yet some common surgical interventions have failure rates as high as 40%. The lack of standard animal models to investigate this condition impedes progress. We propose the following protocol as a model for uterosacral ligament suspension and in vivo tensile testing.

자궁천골 인대 서스펜션 쥐 모델 개발

Pelvic organ prolapse (POP) is a common pelvic floor disorder (PFD) with the potential to significantly impact a woman's quality of life. Approximately ...