JoVE Logo

Sign In

Taipei Medical University

19 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds
Chen-Jei Tai 1,2, Chia-Lin Li 3, Cheng-Jeng Tai 4,5, Chien-Kai Wang 2,4, Liang-Tzung Lin 3,6
1Department of Chinese Medicine, Taipei Medical University Hospital, 2Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, 3Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, 4Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, 5Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, 6Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University

Here, we present a protocol that examines specific steps of the viral entry to identify and evaluate novel antiviral agents.

image

Behavior

Conscious and Non-conscious Representations of Emotional Faces in Asperger's Syndrome
Vincent S. C. Chien 1,2, Arthur C. Tsai 1, Han Hsuan Yang 3, Yi-Li Tseng 4, Alexander N. Savostyanov 5,6, Michelle Liou 1,7
1Institute of Statistical Science, Academia Sinica, 2Max Planck Institute for Human Cognitive and Brain Sciences, 3Department of Psychology, Fo Guang University, 4Department of Electrical Engineering, Fu Jen Catholic University, 5State Research Institute of Physiology and Basic Medicine, 6Novosibirsk State University, 7Imaging Research Center, Taipei Medical University

An EEG experimental protocol is designed to clarify the interplay between conscious and non-conscious representations of emotional faces in patients with Asperger's syndrome. The technique suggests that patients with Asperger's syndrome have deficits in non-conscious representation of emotional faces, but have comparable performance in conscious representation with healthy controls.

image

Neuroscience

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
Chun Austin Changou 1,2,3, Reni Ajoy 4,5, Szu-Yi Chou 4,5,6
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, 3Core Facility, Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

This protocol describes a technique for observation of real-time Green Fluorescence Protein (GFP) tagged Glucose Transporter 4 (GLUT4) protein trafficking upon insulin stimulation and characterization of the biological role of CCR5 in the insulin–GLUT4 signaling pathway with Deconvolution Microscopy.

image

Medicine

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
Reni Ajoy 1,2, Szu-Yi Chou 1,2,3
1The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research, 2Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 3TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.

image

Biology

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
Wan-Shan Yang 1, Mel Campbell 2, Hsing-Jien Kung 2,3,4,5, Pei-Ching Chang 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

Unlike ubiquitin ligases, few E3 SUMO ligases have been identified. This modified in vitro SUMOylation protocol is able to identify novel SUMO E3 ligases by an in vitro reconstitution assay.

image

Medicine

Treatment of Liver Metastases Using an Internal Target Volume Method for Stereotactic Body Radiotherapy
Wei-Jun Wang 1,2, Jeng-Fong Chiou 1,2,3, Yaoru Huang 1,2,4
1Department of Radiation Oncology, Taipei Medical University Hospital, 2Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, 3Taipei Cancer Center, Taipei Medical University, 4Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University

Stereotactic body radiotherapy (SBRT) requires rigorous accuracy and precision for delivering high radiation doses per fraction to small treatment volumes to improve tumor control and simultaneously reduce toxicity. Herein, we present a noninvasive and clinically convenient respiratory motion management protocol for SBRT for liver metastases.

image

Bioengineering

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Yen-Li Wang 1,2, Adrienne Hong 3, Tzung-Hai Yen 1,4,5,6, Hsiang-Hsi Hong 1,7,8
1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.

image

Medicine

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
Chun-Nin Lee 1, Li-Hsuan Chiu 2,5, Chia-Lang Fang 3, Shauh-Der Yeh 4, Chun S. Zuo 5, Shih-Ching Chen 6, Li-Kuo Kuo 7, Yun-Ming Wang 8, Wen-Fu Thomas Lai 2,5,9
1Department of Pulmonary and Critical Care Medicine, Taipei Medical University-Shuang Ho Hospital, 2Department of Research and Department of Dentistry, Taipei Medical University / Shuang-Ho Hospital, 3Department of Pathology, Taipei Medical University, 4Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 5McLean Imaging Center, McLean Hospital/Harvard Medical School, 6Department of Physical Medicine and Rehabilitation, School of Medicine, College of Medicine, Taipei Medical University, 7Department of Medicine, Mackay Medical College, 8Department of Biological Science and Technology, National Chiao Tung University, 9Graduate Institute of Clinical Medicine, Taipei Medical University

To improve serological diagnostic tests for Mycobacterium tuberculosis antigens, we developed superparamagnetic iron oxide nanoprobes to detect extrapulmonary tuberculosis.

image

Medicine

Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus
Jing Liu 1,2, Roberto Copetti 3, Erich Sorantin 4, Jovan Lovrenski 5, Javier Rodriguez-Fanjul 6, Dalibor Kurepa 7, Xing Feng 8, Luigi Cattaross 9, Huayan Zhang 10,11, Misun Hwang 12, Tsu F. Yeh 13,14, Yisrael Lipener 7, Abhay Lodha 15, Jia-Qin Wang 16, Hai-Ying Cao 2,17, Cai-Bao Hu 2,18, Guo-Rong Lyu 19, Xin-Ru Qiu 1,2, Li-Qun Jia 20, Xiao-Man Wang 20, Xiao-Ling Ren 1,2, Jiu-Ye Guo 1,2, Yue-Qiao Gao 1,2, Jian-Jun Li 1,2, Ying Liu 1,2, Wei Fu 1,2, Yan Wang 21, Zu-Lin Lu 1,2, Hua-Wei Wang 8, Li-Li Shang 22
1Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, 2The Neonatal Lung Ultrasound Training Base, Chinese College of Critical Ultrasound, 3Emergency Department, Cattinara University Hospital, 4Division of Pediatric Radiology, Department of Radiology, Medical University Graz, 5Faculty of Medicine, University of Novi Sad, Radiology Department, Institute for Children and Adolescents Health Care of Vojvodina, 6Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, 7Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, 8Department of Neonatology, Children's Hospital of Soochow University, 9Department of Neonatology, Udine University Hospital, 10Center for Newborn Care, Guangzhou Women and Children's Medical Center, 11Division of Neonatology, Children's Hospital of Philadelphia, 12Department of Radiology, Children's Hospital of Philadelphia, 13Division of Neonatology and NICU, Cook County Children's Hospital, University of Illinois, 14Division of Neonatology, Department of Pediatrics, Taipei Medical University, 15Department of Pediatrics and Community Health Sciences, University of Calgary, 16Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical University, 17Department of Ultrasound, GE Healthcare, 18Intensive Care Unit, Zhejiang Hospital, 19Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, 20Department of Ultrasound, Beijing Children's Hospital Affiliated with Capital Medical University, 21Department of Neonatology and NICU, Tai'an City Central Hospital of Shandong Province, 22Department of Intensive Care Unit, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical decision-making.

image

Medicine

Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion
Cheng-Yu Chen *1,2,3,4,5, Ray-Jade Chen *6,7, Gilbert Aaron Lee 5
1Research Center of Translational Imaging, College of Medicine, Taipei Medical University, 2Radiogenomic Research Center, Taipei Medical University Hospital, Taipei Medical University, 3Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, 4Department of Medical Imaging, Taipei Medical University Hospital, Taipei Medical University, 5Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University, 6Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, 7Division of General Surgery, Department of Surgery, Taipei Medical University Hospital

A mouse model of cerebral ischemia-reperfusion is established to investigate the pathophysiology of stroke. We distally ligate the right middle cerebral artery and right common carotid artery and restore blood flow after 10 or 40 min of ischemia.

image

Immunology and Infection

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Ting-Jing Shen 1,2, Ming-Kai Jhan 1,2, Jo-Chi Kao 1,2, Min-Ru Ho 1,2, Tsung-Ting Tsai 1,2, Po-Chun Tseng 1,2, Yung-Ting Wang 1,2, Chiou-Feng Lin 1,2,3
1Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 2Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, 3Center of Infectious Disease and Signaling Research, National Cheng Kung University

Here, we present a protocol for creating an immunocompetent ICR (Institute of Cancer Research) murine model of central nervous system infection to display the development of neuropathy. Monitoring acute viral encephalitic disorders by identical disease scores could be performed for showing dengue virus-induced neuropathy in vivo.

image

Immunology and Infection

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16
Jonathan Y. Wang 1, Chien-Ju Lin 2, Ching-Hsuan Liu 3,4, Liang-Tzung Lin 3,5
1Department of Molecular Biosciences, University of Texas at Austin, 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 3Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 4Department of Microbiology and Immunology, Dalhousie University, 5Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University

The goal of the protocol is to illustrate the different assays relating to viral entry that can be used to identify candidate viral entry inhibitors.

image

Cancer Research

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
Chi-Hao Hsiao 1,2,3, Ya-Hsu Chiu 4, Shao-Chih Chiu 4,5, Der-Yang Cho 4,6,7, Liang-Ming Lee 1,2, Yu-Ching Wen 1,2, Jia-Jhe Li 4, Ping-Hsiao Shih 4
1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.

image

Medicine

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
Rami Ahmad Shahror 1,2,3, Chung-Che Wu 2,3,4,5, Yung-Hsiao Chiang 1,2,3,4,5, Kai-Yun Chen 1,2,3
1Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research Institutes, 2Center for Neurotrauma and Neuroregeneration, Taipei Medical University, 3TMU Neuroscience Research Center, Taipei Medical University, 4Department of Neurosurgery, Taipei Medical University Hospital, 5Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University

Presented here is a protocol for non-invasive mesenchymal stem cell (MSC) delivery and tracking in a mouse model of traumatic brain injury. Superparamagnetic iron oxide nanoparticles are employed as a magnetic resonance imaging (MRI) probe for MSC labeling and non-invasive in vivo tracking following intranasal delivery using real-time MRI.

image

Medicine

Real-Time Magnetic Resonance Guided Focused Ultrasound for Painful Bone Metastases
Wei-Jun Wang 1, Hsin-Lun Lee 1,2, Shiu-Chen Jeng 1,3, Jeng-Fong Chiou 1,4,5, Yaoru Huang 1,6
1Department of Radiation Oncology, Taipei Medical University Hospital, 2The Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei Medical University, 3School of Dentistry, College of Oral Medicine, Taipei Medical University, 4Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, 5Taipei Cancer Center, Taipei Medical University, 6Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University

Magnetic resonance could offer real-time monitoring of the position and temperature of focused ultrasound in thermal ablation for painful bone metastases, regardless of cancer type or previous local treatments. Our innovative method of quality assurance could facilitate the application of this effective and safe treatment.

image

Medicine

Sleeve Gastrectomy in Mice using Surgical Clips
Jih-Hua Wei 1,2,3, Che-Hung Yeh 4, Wei-Jei Lee 5, Shing-Jong Lin 3,6,7,8,9, Po-Hsun Huang 3,6,7
1Cardiovascular Division, Internal Medicine Department, Min-Sheng General Hospital, 2Department of Nutrition and Health Sciences, School of Healthcare Management, Kai-Nan University, 3Institute of Clinical Medicine, National Yang-Ming University, 4Food Science and Biotechnology, College of Biotechnology and Bioresources, University of Da-Yeh, 5Department of Surgery, Min-Sheng General Hospital, 6Department of Critical Care Medicine, Taipei Veterans General Hospital, 7Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, 8Division of Cardiology, Heart Center, Cheng-Hsin General Hospital, 9Taipei Medical University

The prevalence of diabetes and obesity is continuously increasing worldwide. The mechanisms between diabetes, obesity and their associated mortality and co-morbidities need to be further investigated. Here, we present a protocol for sleeve gastrectomy (SG) in animals as an uncomplicated preclinical model of bariatric surgery.

image

Medicine

Intratracheal Instillation of Stem Cells in Term Neonatal Rats
Chung-Ming Chen 1,2, Yue-Jun Chen 1, Zheng-Hao Huang 1
1Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, 2Department of Pediatrics, Taipei Medical University Hospital

Described is a protocol for performing intratracheal transplantation of mesenchymal stromal cells (MSCs) through intratracheal injection in term neonatal rats. This technique is a clinically viable option for delivery of stem cells and drugs into neonatal rat lungs to evaluate their efficacy.

image

Developmental Biology

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
Chuang-Yu Lin 1, Michiko Yoshida 1,2, Li-Tzu Li 3, Megumu K. Saito 1
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

image

Medicine

Left Anterior Descending Coronary Artery Ligation for Ischemia-Reperfusion Research: Model Improvement via Technical Modifications and Quality Control
Hui-Chun Ku 1, Ding-Kuo Chien 2,3,4,5, Chuan-Lei Chao 5,6, Shih-Yi Lee 5,7
1Department of Life Science, Fu Jen Catholic University, 2Department of Medicine, MacKay Medical College, 3Department of Emergency Medicine, MacKay Memorial Hospital, 4Graduate Institute of Injury Prevention and Control, Taipei Medical University, 5MacKay Junior College of Medicine, Nursing, and Management, 6Division of Cardiology, Department of Internal Medicine, Taitung Mackay Memorial Hospital, 7Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, MacKay Memorial Hospital

Herein, we present a protocol focusing on the quality control of the left anterior descending coronary artery ligation by technically modifying the traditional procedure in rats for acute myocardial ischemia-reperfusion research.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved