In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

9.4K Views

09:45 min

January 29th, 2018

DOI :10.3791/56629-v

January 29th, 2018

Transcript

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Keywords In Vitro SUMOylation

Chapters in this video

0:05

Title

0:39

Purification of K-bZIP

3:26

SUMOylation Assay

7:15

Results: Use of In Vitro SUMOylation Assay to Study the Role of SUMO E3 Ligase in p53 SUMOylation Using Optimal Ubc9 Enzyme Concentration

8:49

Conclusion

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