Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

9.6K Views

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08:47 min

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December 7th, 2017

DOI :

10.3791/56409-v

December 7th, 2017

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Chun Austin Changou¹^,²^,³, Reni Ajoy⁴^,⁵, Szu-Yi Chou⁴^,⁵^,⁶

¹The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, ²Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, ³Core Facility, Taipei Medical University, ⁴The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, ⁵Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, ⁶TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

Summary

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Keywords GLUT4

Protein Trafficking

Mouse

Primary Hypothalamic Neurons

Deconvolution Microscopy

Brain Tissue Isolation

Hypothalamus

Brain Regions

Wash Medium

Chapters in this video

0:05

Title

1:00

Primary Neuron Culture and Brain Tissue Isolation

2:56

Tissue Digestion, Plating, and Culture

4:32

Live Image Recording

6:48

Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons

7:47

Conclusion

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