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Mississippi State University

14 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation and Derivation of Mouse Embryonic Germinal Cells
Harold Moreno-Ortiz 1, Clara Esteban-Perez 1, Wael Badran 1, Marijo Kent-First 1
1Reproductive Genetics and Stem Cell Laboratory, Department of Biological Sciences, Mississippi State University

The ability of embryonic germinal cells to differentiate into primordial germinal cells during early development stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum mouse embryos.

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Immunology and Infection

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
Simon Menanteau-Ledouble 1, Mark Lawrence 1
1Department of Basic Sciences, Mississippi State University

Here we describe a procedure allowing the labeling of Edwardsiella ictaluri in situ in histological sections from channel catfish Ictalurus punctatus using indirect immunohistochemistry with monoclonal antibodies Ed9 as a primary, and fluorescent FitC labeled antibodies as a secondary. This allowed for the detection of the bacterium using fluorescent microscopy.

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JoVE Core

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
Kimberly J. Schipke 1, S. D. Filip To 1, James N. Warnock 1
1Department of Agricultural and Biological Engineering, Mississippi State University

A cyclic pressure bioreactor capable of subjecting heart valve tissue to physiological and pathological pressure conditions has been designed. A LabVIEW program allows users to control pressure magnitude, amplitude and frequency. This device can be used to study the mechanobiology of heart valve tissue or isolated cells.

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Bioengineering

A Coupled Experiment-finite Element Modeling Methodology for Assessing High Strain Rate Mechanical Response of Soft Biomaterials
Rajkumar Prabhu 1, Wilburn R. Whittington 2, Sourav S. Patnaik 1, Yuxiong Mao 2, Mark T. Begonia 1, Lakiesha N. Williams 1, Jun Liao 1, M. F. Horstemeyer 2
1Department of Agricultural and Biological Engineering, Mississippi State University, 2Center for Advanced Vehicular Systems, Mississippi State University

The current study prescribes a coupled experiment-finite element simulation methodology to obtain the uniaxial dynamic mechanical response of soft biomaterials (brain, liver, tendon, fat, etc.). The multiaxial experimental results that arose because of specimen bulging obtained from Split-Hopkinson Pressure Bar testing were rendered to a uniaxial true stress-strain behavior when simulated through iterative optimization of the finite element analysis of the biomaterial.

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JoVE Core

Modified Drop Tower Impact Tests for American Football Helmets
G. Alston Rush 1,2, R. Prabhu 1, Gus A. Rush III 3, Lakiesha N. Williams 1, M. F. Horstemeyer 2,4
1Agricultural and Biological Engineering, Mississippi State University, 2Center for Advanced Vehicular Systems, Mississippi State University, 3Rush Sport Medical, 4Mechanical Engineering, Mississippi State University

This article provides a novel technique to assess the performance characteristics of American football helmets by inclusion of faceguards during NOCSAE Standard drop tests. Additionally, two more impact locations are proposed to be added to the NOCSAE certification.

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JoVE Journal

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
Ryan C. Range 1, Marina Martinez-Bartolomé 1, Stephanie D. Burr 1
1Department of Biological Sciences, Mississippi State University

This video article details a straightforward in vivo methodology that can be used to systematically and efficiently characterize components of complex signaling pathways and regulatory networks in many invertebrate embryos.

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Education

Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
Eric S. Michel 1,2, Emily B. Flinn 1, Stephen Demarais 1, Bronson K. Strickland 1, Guiming Wang 1, Chad M. Dacus 3
1Department of Wildlife, Fisheries and Aquaculture, Mississippi State University, 2Department of Natural Resource Management, South Dakota State University, 3Mississippi Department of Wildlife, Fisheries and Parks

Phenotypic differences among cervid populations may be related to population-level genetics or nutrition; discerning which is difficult in the wild. This protocol describes how we designed a controlled study where nutritional variation was eliminated. We found that phenotypic variation of male white-tailed deer was more limited by nutrition than genetics.

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Genetics

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Clark Fritsch 1,2, Jean-Francois Pierre Gout 3,4, Marc Vermulst 1
1Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia, 2Department of Cellular and Molecular Biology, University of Pennsylvania, 3Department of Biological Sciences, Mississippi State University, 4Center for Mechanisms of Evolution, Biodesign Institute, Arizona State University

This protocol provides researchers with a new tool to monitor the fidelity of transcription in multiple model organisms.

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Biology

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice (Oryza sativa ssp.)
Brooklyn C. Schumaker 1, Shandrea Stallworth 1, Edicarlos De Castro 1, Mary Grace Fuller 1, Swati Shrestha 1, Te-Ming Tseng 1
1Department of Plant and Soil Science, Mississippi State University

Allelopathy has shown promise as a useful supplemental weed control strategy in cropping systems. To determine the allelopathic potential of a desired plant specimen, a stair-step screening method is provided.

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Genetics

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information
Adam Thrash 1, Marilyn L Warburton 2
1Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, 2Corn Host Plant Resistance Research Unit, USDA-ARS

By running the Pathway Association Study Tool (PAST), either through the Shiny application or through the R console, researchers can gain a deeper understanding of the biological meaning of their genome-wide association study (GWAS) results by investigating the metabolic pathways involved.

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Biology

A Cost Effective and Adaptable Scratch Migration Assay
Stephanie D. Burr 1, James A Stewart, Jr. 1
1Department of BioMolecular Sciences, University of Mississippi

We present a cost-effective method to the scratch migration assay that provides a new approach for determining cell migration without the use of equipment-intensive methods. While fibroblasts were used in this protocol, it can be adapted and utilized to study additional cell types and influences on cell migration.

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Biology

Embryo Rescue Protocol for Interspecific Hybridization in Squash
Yuqing Fu 1, Swati Shrestha 1, Pamela Moon 1, Geoffrey Meru 1
1UF/IFAS Tropical Research and Education Center

The article describes an embryo rescue protocol for the regeneration of immature embryos derived from the interspecific hybridization of Cucurbita pepo and Cucurbita moschata. The protocol can be easily replicated and will be an important resource for squash breeding programs.

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Medicine

Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
Lucie Henein 1,2, Randy Clevenger 1, Karen Keeran 1, Lauren Brinster 3
1Animal Surgery and Resources Core, National Heart, Lung, and Blood Institute, National Institutes of Health, 2College of Veterinary Medicine, Mississippi State University, 3Division of Veterinary Resources, Office of Research Services, National Institutes of Health

We describe how to generate a widely used surgical model of intestinal ischemia-reperfusion injury (IRI) in rodents. The procedure involves occlusion of the superior mesenteric artery followed by the restoration of blood flow. This model is useful for studies investigating occlusive causes of intestinal IRI in both veterinary and human medicine.

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Biology

Evaluating the Effects of Biotoxins on Immune Cell Functions in Zebrafish
Lora Petrie-Hanson 1, Ann E. Beth Peterman 1
1Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University

This protocol describes flow cytometric assays that can evaluate the effects of toxin exposure on the endocytic functions of different subpopulations of zebrafish leukocytes. The use of specific functional inhibitors in the assay allows differentiation of the altered endocytic mechanisms.

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