RNA Circularization and Rolling Circle Reverse Transcription
2:51
Second Strand cDNA Synthesis and Repair
3:45
Adapter Ligation and Size Selection
6:21
PCR Amplification and Final Bead Purification
7:53
Results: Quality Control, Bio-Informatic Analysis, and Expected Results from the C-seq Assay
8:54
Conclusion
Transcript
This method can help answer key questions in the field of transcriptional mutagenesis such as which RNA polymerase subunits or biological processes control the fidelity of transcription. The main advantage of this technique is that it allows for t
Sign in or start your free trial to access this content
This protocol provides researchers with a new tool to monitor the fidelity of transcription in multiple model organisms.