Beihang University

13 ARTICLES PUBLISHED IN JoVE

JoVE Core

Preparation and High-temperature Anti-adhesion Behavior of a Slippery Surface on Stainless Steel

Pengfei Zhang¹, Chen Huawei¹, Guang Liu¹, Liwen Zhang¹, Deyuan Zhang¹

¹School of Mechanical Engineering and Automation, Beihang University

Slippery surfaces provide a new way to solve the adhesion problem. This protocol describes how to fabricate slippery surfaces at high temperatures. The results demonstrate that the slippery surfaces showed anti-wetting for liquids and a remarkable anti-adhesion effect on soft tissues at high temperatures.

Developmental Biology

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Qinggang Dai^*1, Yujiao Han^*2, Furong Xie^*1, Xuhui Ma³, Zhan Xu², Xiao Liu¹, Weiguo Zou², Jun Wang¹

¹Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, ²State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, ³Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine

This manuscript describes a protocol to isolate and culture osteoclasts in vitro from mouse bone marrow, and to study the role of the mammalian/mechanistic target of rapamycin complex 1 in osteoclast formation.

JoVE Journal

A 100 KW Class Applied-field Magnetoplasmadynamic Thruster

Baojun Wang¹, Haibin Tang², Yibai Wang¹, Chao Lu¹, Cheng Zhou³, Yangyang Dong¹, Ge Wang³, Yuntian Cong³, Daniel Luu⁴, Jinbin Cao²

¹Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Astronautics, Beihang University, ²Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Space and Environment, Beihang University, ³Beijng Institute of Control Engineering, ⁴School of Aerospace Engineering and Geodesy, University of Stuttgart

The goal of this protocol is to introduce the design of a 100 kW class applied-field magnetoplasmadynamic thruster and relevant experimental methods.

JoVE Journal

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Grant A. R. Gale^*1,2,3, Alejandra A. Schiavon Osorio^*1,2, Anton Puzorjov^*1,2, Baojun Wang^2,3, Alistair J. McCormick^1,2

¹Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, ²Centre for Synthetic and Systems Biology, University of Edinburgh, ³Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh

Here, we present a protocol describing how to i) assemble a self-replicating vector using the CyanoGate modular cloning toolkit, ii) introduce the vector into a cyanobacterial host by conjugation, and iii) characterize transgenic cyanobacteria strains using a plate reader or flow cytometry.

Environment

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell

Liyi Wang^1,2, Nana Xue^1,2, Wenfeng Li², Rehemanjiang Wufuer¹, Daoyong Zhang^1,3

¹Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology

This paper introduces a method of hatching without using an eggshell for toxicological studies of particle pollutants such as microplastics.

Bioengineering

Microfluidic Model to Mimic Initial Event of Neovascularization

Ping Zhao¹, Xing Zhang¹, Xiao Liu¹, Li Wang⁴, Haoran Su¹, Liyi Wang¹, Dongrui Zhang¹, Xiaoyan Deng³, Yubo Fan^1,2

¹Beijing Advanced Innovation Centre for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology of Chinese Education Ministry, School of Biological Science and Medical Engineering, Beihang University, ²School of Engineering Medicine, Beihang University, ³Artificial Intelligence Key Laboratory of Sichuan Province, School of Automation and Information Engineering, Sichuan University of Science and Engineering, ⁴Beijing Research Center of Urban System Engineering

Here, we provide a microfluidic chip and an automatically controlled, highly efficient circulation microfluidic system that recapitulates the initial microenvironment of neovascularization, allowing endothelial cells (ECs) to be stimulated by high luminal shear stress, physiological level of transendothelial flow, and various vascular endothelial growth factor (VEGF) distribution simultaneously.

Medicine

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

Decheng Yin^*1,2, Jian Fu^*1,3, Ida Allabauer¹, Oliver Witzke⁴, Song Rong⁵, André Hoerning¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, ⁵Department of Nephrology, Hannover Medical School

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

Biology

Microsurgical Obstruction of Testes Fusion in Spodoptera litura

Xiaolin He^*1, Qianqian Ma^*1, Baozhu Jian¹, Yucheng Liu¹, Qiong Wu¹, Meixin Chen¹, Qili Feng¹, Ping Zhao², Lin Liu¹

¹South China Normal University-Shipai Campus: South China Normal University, ²Southwest University

Aluminum foil was microsurgically inserted between the testes of Spodoptera litura to obstruct the fusion of testis. The procedure includes freezing, fixing, disinfection, incision, placing the barrier, suturing, postoperative feeding, and inspection. This approach provides a method to interfere with tissue formation.

Immunology and Infection

A Modified Surgical Technique for Kidney Transplantation in Mice

Decheng Yin^*1,2, Jian Fu^*1,3, Rongjun Chen⁴, Nelli Shushakova⁵, Ida Allabauer¹, Xin-Yi Wei¹, Mario Schiffer⁶, Diana Dudziak⁷, Song Rong^*5, André Hoerning^*1

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, ⁵Department of Nephrology, Hannover Medical School, ⁶Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ⁷Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg

This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.

Engineering

Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators

Jiangao Zhao^1,2, Deming Zhu^2,3, Yuxuan Ma^2,3, Yongling Fu^2,3, Jian Fu^2,3

¹Research Institute for Frontier Science, Beihang University, ²School of Mechanical Engineering and Automation, Beihang University, ³Laboratory of Aerospace Servo Actuation and Transmission, Beihang University

We built a simulation model to evaluate pump flow characteristics and performance of the single-shaft coaxial motor-pump assembly in electrohydrostatic actuators and investigate the overall efficiency in a wide set of working conditions of the motor-pump assembly experimentally.

JoVE Core

Design and Application of a Fault Detection Method Based on Adaptive Filters and Rotational Speed Estimation for an Electro-Hydrostatic Actuator

Yongling Fu^1,2, Yuxuan Ma^1,2, Zhenyu Gou³, Tuanhui Guo^1,2, Junhao Liu^1,2, Jiangao Zhao^2,4

¹School of Mechanical Engineering and Automation, Beihang University, ²Laboratory of Aerospace Servo Actuation and Transmission, Beihang University, ³AVIC Chengdu Aircraft Design & Research Institute, ⁴Research Institute for Frontier Science, Beihang University

In this paper, an adaptive filter based on a normalized least mean square (NLMS) algorithm and a rotational speed estimation method are introduced to detect the electrical and hydraulic faults of the electro-hydrostatic actuator (EHA). The efficacy and feasibility of the aforementioned methods are verified through simulations and experiments.

JoVE Core

A Modeling and Simulation Method for Preliminary Design of an Electro-Variable Displacement Pump

Xu Han¹, Peng Zhang², Tatiana Minav³, Yongling Fu¹, Jian Fu¹

¹School of Mechanical Engineering and Automation, Beihang University, ²Beijing Institute of Precision Mechatronics and Controls, ³Innovative Hydraulics and Automation, Tampere University

A simulation model specifically supporting the preliminary design of an electro-variable displacement pump (EVDP) is developed and partially verified by experiments. The control performance, life, reliability, etc., can all be evaluated using the proposed model, which covers the main performance requirements under the EVDP preliminary design task.

Medicine

Augmented Reality Navigation-Guided Core Decompression for Osteonecrosis of Femoral Head

Qi Wang^*1,3, Qiuyuan Wang^*2,3, Ran Ding³, Youjie Yao⁴, Junjun Pan⁵, Weiguo Wang³

¹China-Japan Friendship School of Clinical Medicine, Peking University, ²Graduate School of Beijing University of Chinese Medicine, ³Department of Orthopedic Surgery, Beijing Key Lab of Immunes-Mediated Inflammatory Disease, China-Japan Friendship Hospital, ⁴Beijing Normal University, ⁵State Key Laboratory of Virtual Reality Technology and Systems, Beihang University

Augmented reality technology was applied to core decompression for osteonecrosis of the femoral head to realize real-time visualization of this surgical procedure. This method can effectively improve the safety and precision of core decompression.