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A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

12.7K Views

09:37 min

March 15th, 2018

DOI :

10.3791/56468-v

March 15th, 2018


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Keywords Osteoclast

Chapters in this video

0:05

Title

0:59

Dissection and Isolation

3:27

Plating and Differentiation

5:41

Tartrate Resistant Acid Phosphatase Staining and Activity Quantification of Culture Medium

7:25

Results: Deletion of Raptor Impaired Osteoclast Formation

8:27

Conclusion

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