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University of Twente

16 ARTICLES PUBLISHED IN JoVE

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Biology

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
Stefan Giselbrecht 1, Eric Gottwald 1, Roman Truckenmueller 2, Christina Trautmann 3, Alexander Welle 1, Andreas Guber 4, Volker Saile 4, Thomas Gietzelt 5, Karl-Friedrich Weibezahn 1
1Institute for Biological Interfaces, Karlsruhe Research Centre, 2Institute for BioMedical Technology, University of Twente, 3Department of Materials Research, Institute for Heavy Ion Research, 4Institute of Microstructure Technology, Karlsruhe Research Centre, 5Institute for Micro Process Engineering, Karlsruhe Research Centre

We present two processes for the microfabrication of porous polymer chips for three-dimensional cell cultivation. The first one is hot embossing combined with a solvent vapour welding process. The second one uses a recently developed microthermoforming process combined with ion track technology leading to a significant simplification of manufacture.

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Engineering

Preparation and Use of Photocatalytically Active Segmented Ag|ZnO and Coaxial TiO2-Ag Nanowires Made by Templated Electrodeposition
A. Wouter Maijenburg 1, Eddy J.B. Rodijk 1, Michiel G. Maas 1, Johan E. ten Elshof 1
1MESA+ Institute for Nanotechnology, University of Twente

Procedures are outlined to prepare segmented and coaxial nanowires via templated electrodeposition in nanopores. As examples, segmented nanowires consisting of Ag and ZnO segments, and coaxial nanowires consisting of a TiO2 shell and a Ag core were made. The nanowires were used in photocatalytic hydrogen formation experiments.

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Engineering

Coherent anti-Stokes Raman Scattering (CARS) Microscopy Visualizes Pharmaceutical Tablets During Dissolution
Andrew L. Fussell 1, Peter Kleinebudde 2, Jennifer Herek 1, Clare J. Strachan 3, Herman L. Offerhaus 1
1Optical Sciences Group, MESA+ Institute, University of Twente, 2Institute of Pharmaceutics and Biopharmaceutics, Heinrich-Heine University, 3Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki

Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with an intrinsic flow-through dissolution setup to allow in situ and real-time visualization of the surface of pharmaceutical tablets undergoing dissolution. Using this custom-built setup, it is possible to correlate CARS videos with drug dissolution profiles recorded using inline UV absorption spectroscopy.

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Chemistry

Atomically Defined Templates for Epitaxial Growth of Complex Oxide Thin Films
A. Petra Dral 1, David Dubbink 1, Maarten Nijland 1, Johan E. ten Elshof 1, Guus Rijnders 1, Gertjan Koster 1
1MESA+ Institute for Nanotechnology, University of Twente

Various procedures are outlined to prepare atomically defined templates for epitaxial growth of complex oxide thin films. Chemical treatments of single crystalline SrTiO3 (001) and DyScO3 (110) substrates were performed to obtain atomically smooth, single terminated surfaces. Ca2 Nb3 O10- nanosheets were used to create atomically defined templates on arbitrary substrates.

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Engineering

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
Sissi de Beer 1,2, Edit Kutnyanszky 2, Martin H. Müser 1,3, G. Julius Vancso 2
1Jülich Supercomputing Centre, Forschungszentrum Jülich, 2Materials Science and Technology of Polymer, MESA+ Institute for Nanotechnology, University of Twente, 3Department of Materials Science and Engineering, Universität des Saarlandes

The methodology to perform friction force microscopy experiments for contacting brushes is presented: Two polymer brushes that are grafted from (a) substrates and (b) colloidal probes are slid to show that, by using two contacting immiscible brush systems, friction in sliding contacts is reduced compared to miscible brush systems.

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Chemistry

Dynamic Electrochemical Measurement of Chloride Ions
Yawar Abbas 1, Derk B. de Graaf 1, Wouter Olthuis 1, Albert van den Berg 1
1BIOS-Lab on a chip group, MESA+ Institute of Nanotechnology, MIRA Biomedical Technology and Technical Medicine, University of Twente

Dynamic measurement of chloride ions is presented. Transition time of an Ag/AgCl electrode, during a chronopotentiometric technique, can give the concentration of chloride ions in electrolyte. This method does not require a stable conventional reference electrode.

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Developmental Biology

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
Luca Sala 1, Dorien Ward-van Oostwaard 1, Leon G. J. Tertoolen 1, Christine L Mummery 1,2, Milena Bellin 1
1Department of Anatomy and Embryology, Leiden University Medical Center, 2Department of Applied Stem Cell Technologies, University of Twente

Electrophysiological characterization of cardiomyocytes derived from human Pluripotent Stem Cells (hPSC-CMs) is crucial for cardiac disease modeling and for determining drug responses. This protocol provides the necessary information to dissociate and plate hPSC-CMs on multi-electrode arrays, measure their field potential, and a method for analyzing QT and RR intervals.

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Bioengineering

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
Marinke W. van der Helm 1, Mathieu Odijk 1, Jean-Philippe Frimat 1,2, Andries D. van der Meer 3, Jan C.T. Eijkel 1, Albert van den Berg 1, Loes I. Segerink 1
1BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics, University of Twente, 2Microsystems, Eindhoven University of Technology, 3Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente

This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.

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Bioengineering

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection
Xiaolin Zhang 1,2, Leonie de Boer 1, Oliver W. Stockhammer 1, Dirk W. Grijpma 2,3, Herman P. Spaink 4, Sebastian A.J. Zaat 1
1Department of Medical Microbiology, Amsterdam UMC, 2Technical Medical Center, Department of Biomaterials Science and Technology, University of Twente, 3Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen, University of Groningen, 4Institute of Biology, Leiden University

The present study describes a zebrafish embryo model for in vivo visualization and intravital analysis of biomaterial-associated infection over time based on fluorescence microscopy. This model is a promising system complementing mammalian animal models such as mouse models for studying biomaterial-associated infections in vivo.

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Developmental Biology

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
Oleh V. Halaidych 1, Francijna van den Hil 1, Christine L. Mummery 1,2, Valeria V. Orlova 1
1Department of Anatomy and Embryology, Leiden University Medical Center, 2Department of Applied Stem Cell Technologies, University of Twente

This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow.

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Engineering

Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies
Didier Goedertier 1, Sandra Schorderet Weber 1, Francesco Lucci 1, Tom Lee 2, Wei Teck Tan 2, Falk Radtke 1, Subash Krishnan 2, Patrick Vanscheeuwijck 1, Arkadiusz K. Kuczaj 1,3, Julia Hoeng 1
1PMI R&D, Philip Morris Products S.A., 2PMI R&D, Philip Morris Research Laboratories Pte. Ltd., 3Applied Mathematics, University of Twente

The protocol describes the settings and use of a capillary aerosol generator for continuous production of controlled aerosol from a multispecies liquid solution, suitable for steady large-volume aerosol delivery (e.g., in vivo inhalation studies).

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Immunology and Infection

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
Xue D. Manz 1, Hugo J. Albers 2,3, Petr Symersky 4, Jurjan Aman 1, Andries D. van der Meer 3, Harm Jan Bogaard 1, Robert Szulcek 1,5,6
1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, 5Institute of Physiology, Charité-Universitätsmedizin, 6German Heart Center

We present an in vitro vascular disease model to investigate whole blood interactions with patient-derived endothelium. This system allows the study of thrombogenic properties of primary endothelial cells under various circumstances. The method is especially suited to evaluate in situ thrombogenicity and anticoagulation therapy during different phases of coagulation.

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Bioengineering

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
Charlotte Nawijn 1, Tim Segers 1,2, Guillaume Lajoinie 1, Ýrr Mørch 3, Sigrid Berg 4,5,6, Sofie Snipstad 3,6,7, Catharina de Lange Davies 7, Michel Versluis 1
1Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 2BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 3Department of Biotechnology and Nanomedicine, SINTEF Industry, 4Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, 5Department of Health Research, SINTEF Digital, 6Cancer Clinic, St. Olav’s Hospital, 7Department of Physics, Norwegian University of Science and Technology

The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and timescales.

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Engineering

Preparation of Free-Surface Hyperbolic Water Vortices
Roman Klymenko 1,5, Harmen Nanninga 2, Esther de Kroon 1, Luewton L. F. Agostinho 1,2, Elmar C. Fuchs 1,3, Jakob Woisetschläger 4, Wilfred F. L. M. Hoeben 5
1Wetsus - Centre of Excellence for Sustainable Water Technology, 2Water Technology Research Group, NHL Stenden University of Applied Sciences, 3Optical Sciences Group, Faculty of Science and Technology (TNW), University of Twente, 4Institute for Thermal Turbomachinery and Machine Dynamics, Graz University of Technology, 5Department of Electrical Engineering, Electrical Energy Systems group, Eindhoven University of Technology

This paper describes how three different water vortex regimes in a hyperbolic Schauberger funnel can be created, their most important characteristics, and how associated parameters such as the oxygen transfer rates can be calculated.

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Neuroscience

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
Lena Sophie Koch 1, David Choy Buentello 2,3, Kerensa Broersen 1
1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School

The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

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Medicine

Reduction of Radiation Exposure during Endovascular Treatment of Peripheral Arterial Disease Combining Fiber Optic RealShape Technology and Intravascular Ultrasound
Constantijn E. V. B. Hazenberg 1, Suzan C. A. Wulms 2, Jurre Klaassen 1, Joost A. van Herwaarden 1
1Department of Vascular Surgery, University Medical Center Utrecht, 2Technical Medicine, University of Twente

Described here is a stepwise method of combining Fiber Optic RealShape technology and intravascular ultrasound to show the potential of merging both techniques, in view of the reduction of radiation exposure and improvement of navigation tasks and treatment success during an endovascular procedure for the treatment of peripheral arterial disease.

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