Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

3.7K Views

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06:02 min

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June 12th, 2021

DOI :

10.3791/62251-v

June 12th, 2021

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Charlotte Nawijn¹, Tim Segers¹^,², Guillaume Lajoinie¹, Ýrr Mørch³, Sigrid Berg⁴^,⁵^,⁶, Sofie Snipstad³^,⁶^,⁷, Catharina de Lange Davies⁷, Michel Versluis¹

¹Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, ²BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, ³Department of Biotechnology and Nanomedicine, SINTEF Industry, ⁴Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, ⁵Department of Health Research, SINTEF Digital, ⁶Cancer Clinic, St. Olav’s Hospital, ⁷Department of Physics, Norwegian University of Science and Technology

Summary

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Multi timescale Microscopy

Fluorescently Labeled Microbubbles

Ultrasound triggered Drug Delivery

Imaging Techniques

Brightfield Microscopy

Microbubble Suspension

Phosphate Buffered Saline

Ultrasound Frequency

Acoustic Pressure

Optical Fiber

Fluorescence Microscopy

Nanoparticle Excitation

Sample Analysis

XYZ Stage

Chapters in this video

0:05

Introduction

0:45

Imaging by Brightfield Microscopy

2:05

Imaging by Fluorescence Microscopy

2:52

Imaging Protocol by Intravital Microscopy

4:07

Results: In Vitro and In Vivo Behavior of Insonified Microbubble

5:22

Conclusion

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