Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

16.1K Views

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10:51 min

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September 26th, 2017

DOI :

10.3791/56334-v

September 26th, 2017

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Marinke W. van der Helm¹, Mathieu Odijk¹, Jean-Philippe Frimat¹^,², Andries D. van der Meer³, Jan C.T. Eijkel¹, Albert van den Berg¹, Loes I. Segerink¹

¹BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics, University of Twente, ²Microsystems, Eindhoven University of Technology, ³Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente

Summary

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Keywords Organ on chip

Transendothelial Electrical Resistance

TEER

Blood brain Barrier

Microfluidic Chip

Integrated Electrodes

PDMS Fabrication

Polycarbonate Membrane

Two layer Device

Barrier Function

Drug Discovery

Personalized Medicine

Lung on chip

Gut on chip

Chapters in this video

0:05

Title

1:11

Fabrication of the Organ-on-chip Device

5:37

On-chip Culture of Brain-derived Endothelial Cells

8:42

Results: Representative Impedence Data, TEER Determination and Development of BBB-on-chip

10:04

Conclusion

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