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Whitehead Institute of Biomedical Research

5 ARTICLES PUBLISHED IN JoVE

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Biology

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
Ellie Graeden 1,2, Hazel Sive 1,2
1Department of Biology, MIT - Massachusetts Institute of Technology, 2Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

In this video, we demonstrate a method by which to analyze the developing vertebrate brain in live zebrafish embryos at single cell resolution by confocal microscopy. This includes the method by which we inject the single-cell zebrafish embryo and subsequently mount and image the developing brain.

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Biology

Zebrafish Brain Ventricle Injection
Jennifer H. Gutzman 1, Hazel Sive 1,2
1Whitehead Institute for Biochemical Research, 2MIT - Massachusetts Institute of Technology

After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.

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Neuroscience

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
Jessica T. Chang 1,2, Hazel Sive 1,2
1Department of Biology, Massachusetts Institute of Technology, 2Whitehead Institute of Biomedical Research

We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.

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Neuroscience

Manual Drainage of the Zebrafish Embryonic Brain Ventricles
Jessica T. Chang 1, Hazel Sive 1
1Department of Biology, Whitehead Institute of Biomedical Research, Massachusetts Institute of Technology

We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.

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Biology

Facial Transplants in Xenopus laevis Embryos
Laura A. Jacox *1,3, Amanda J. Dickinson *4, Hazel Sive 2,3
1Biological Sciences in Dental Medicine, Harvard University, 2Biology Department, Massachusetts Institute of Technology, 3Whitehead Institute, Massachusetts Institute of Technology, 4Biology Department, Virginia Commonwealth University

A technique for transplanting "Extreme Anterior Domain" facial tissue between Xenopus laevis embryos has been developed. Tissue can be moved from one gene expression background into another, allowing the study of local requirements for craniofacial development and for signaling interactions between facial regions.

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