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An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

DOI :

10.3791/4242-v

October 24th, 2012

October 24th, 2012

9,366 Views

1Department of Biology, Massachusetts Institute of Technology, 2Whitehead Institute of Biomedical Research

We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.

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Permeability

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