Leibniz Institute for Farm Animal Biology (FBN)

3 ARTICLES PUBLISHED IN JoVE

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Biology

In Vivo Microinjection and Electroporation of Mouse Testis
Marten Michaelis 1, Alexander Sobczak 1, Joachim M. Weitzel 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.

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Biology

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
Anja Baufeld 1, Jens Vanselow 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

A long-term culture model of bovine granulosa cells under serum-free conditions is described. This model allows researchers to study the effects of diverse factors and conditions as different plating densities on the characteristics of estrogen-producing bovine granulosa cells.

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Biochemistry

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
Louisa Jutta Dietz 1, Anuroop Venkateswaran Venkatasubramani 1, Annika Müller-Eigner 2, Martin Hrabe de Angelis 3,4,5, Axel Imhof 1, Lore Becker 3, Shahaf Peleg 2,6,7
1Munich Center of Integrated Protein Science and Biomedical Center, Ludwig-Maximilians University of Munich, 2Laboratory for Metabolism and Epigenetics in Aging, Leibniz Institute for Farm Animal Biology (FBN), 3German Mouse Clinic, Helmholtz Zentrum Munich, German Research Center for Environment and Health (GmbH), 4German Center for Diabetes Research (DZD), 5Chair of Experimental Genetics, School of Life Science Weihenstephan, Technische Universität München, 6Laboratory for Metabolism and Epigenetics in Brain Aging, Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, 7Molecular Biology Division, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians University of Munich

Measuring alterations in metabolic rates is central to understanding the progression of various diseases and aging. Here, we present a novel technique to measure whole head oxygen consumption that more closely resembles the physiological state and may aid in revealing novel drugs that modify mitochondrial activity.