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In Vivo Microinjection and Electroporation of Mouse Testis

DOI :

10.3791/51802-v

August 23rd, 2014

August 23rd, 2014

26,793 Views

1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.

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Microinjection

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