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Southern University of Science and Technology

16 ARTICLES PUBLISHED IN JoVE

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Biology

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
Xin Zhang *1, Keshava Mysore *2,3, Ellen Flannery 3,4, Kristin Michel 1, David W. Severson 3,4, Kun Yan Zhu 5, Molly Duman-Scheel 2,3,4
1Division of Biology, Kansas State University, 2Department of Medical and Molecular Genetics, Indiana University School of Medicine, 3Eck Institute for Global Health, University of Notre Dame, 4Department of Biological Sciences, University of Notre Dame, 5Department of Entomology, Kansas State University

Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by larvae.

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Medicine

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging
Lei Li *1, Xiaoyun Guo *1, Yi Chen 1, Haifeng Yin 1, Jing Li 1, Jessica Doan 1, Qinghang Liu 1
1Department of Physiology and Biophysics, University of Washington

The goal of this protocol is to noninvasively assess cardiac structural and functional changes in a mouse model of heart disease created by transverse aortic constriction, using B- and M-mode echocardiography and color/pulse wave Doppler imaging.

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Cancer Research

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Yongyang Huang 1, Jinyun Zou 1, Mudabbir Badar 1, Junchao Liu 1, Wentao Shi 5, Shunqiang Wang 2, Qiongyu Guo 3, Xiaofang Wang 1, Sarah Kessel 4, Leo Li-Ying Chan 4, Peter Li 4, Yaling Liu 2,5, Jean Qiu 4, Chao Zhou 1,5,6
1Department of Electrical and Computer Engineering, Lehigh University, 2Department of Mechanical Engineering, Lehigh University, 3Department of Biomedical Engineering, Southern University of Science and Technology, 4Department of Technology R&D, Nexcelom Bioscience LLC, 5Department of Bioengineering, Lehigh University, 6Center for Photonics and Nanoelectronics, Lehigh University

Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated.

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Cancer Research

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
Ravan Moret 1, Linh Hellmers 1, Xin Zhang 1, Jessie Gills 2, Nathan Hite 3, Aaron Klinger 3, Grace A. Maresh 1, Daniel Canter 2,4, Stephen Bardot 2,4, David A Margolin 3,4, Li Li 1,4
1Laboratory of Translational Cancer Research, Institute for Translational Research, Ochsner Clinic Foundation, 2Department of Urology, Ochsner Clinic Foundation, 3Department of Colon and Rectal Surgery, Ochsner Clinic Foundation, 4Ochsner Clinical School, University of Queensland, School of Medicine

This protocol describes the generation of patient-derived orthotopic xenograft models by intra-vesically instilling high-grade urothelial cell carcinoma cells or intra-rectally injecting colorectal cancer cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice for primary tumor growth and spontaneous metastases under the influence of lymph node stromal cells, which mimics the progression of human metastatic diseases.

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Chemistry

Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations
HuiHui Hu 1, DeBen Lu 1, Kun Peng Dou 1, Xing-Qiang Shi 2
1College of Information Science and Engineering, Ocean University of China, 2Department of Physics, Southern University of Science and Technology

Calculations performed by the Vienna Ab initio Simulation Package can be used to identify the intrinsic electronic properties of nanoscale materials and predict the potential water-splitting photocatalysts.

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Neuroscience

WheelCon: A Wheel Control-Based Gaming Platform for Studying Human Sensorimotor Control
Quanying Liu 1,2,3, Yorie Nakahira 2, Zhichao Liang 1, Ahkeel Mohideen 2, Adam Dai 2, Sung Hoon Choi 2, Angelina Pan 2, Dimitar M. Ho 2, John C. Doyle 2
1Department of Biomedical Engineering, Southern University of Science and Technology, 2Division of Engineering and Applied Science, California Institute of Technology, 3Neuroscience Center, Huntington Medical Research Institutes

WheelCon is a novel, free and open-source platform to design video games that noninvasively simulates mountain biking down a steep, twisting, bumpy trail. It contains components presenting in human sensorimotor control (delay, quantization, noise, disturbance, and multiple feedback loops) and allows researchers to study the layered architecture in sensorimotor control.

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Immunology and Infection

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
Xiaoyan Chang *1, Keru Zhou *1, Yanguang Liu 1,2, Lian Duan 3, Lichun Zhang 2, Guoliang Zhang 3, Haikun Wang 4, Guiqin Wang 1
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences

Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity.

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Biochemistry

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates
Siyu Guo 1, Zhao Liu 1, Yongshuai Yang 1, Jun Chen 1, Chun Loong Ho 1
1Department of Biomedical Engineering, Southern University of Science and Technology

Here, we describe the use of inducer gradient plates to evaluate bacterial swarming motility while simultaneously obtaining multiple concentration responses.

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Bioengineering

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
Wanjie Wu 1, Xuesong Li 1, Jianan Y. Qu 1,2,3,4, Sicong He 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice.

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Biology

Measuring the Migration and Biofilm Formation of Various Bacteria
Yuhao Ma 1, Zahariah Hasan 2, Jia Huang 3, Jun Chen 1, Chun Loong Ho 1
1Department of Biomedical Engineering, Southern University of Science and Technology, 2Desa Mahkota Form Six College, Ministry of Education Malaysia, 3Department of Critical Care Medicine, The Second Affiliated Hospital School of Medicine, Southern University of Science and Technology

Here, we present a practical method for the isolation and identification of microorganisms within the host. In this way, the physicochemical properties of microorganisms and possible ways of living in the host are clearly described.

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Behavior

Physical Activity Measurement in Children Accepting Table Tennis Training
Xin Zhang *1, Cuiqiao Xia *2, Xiuli Zhao 3, Yu Liu 3, Hóngyi Zhào 2,3, Yonghua Huang 2
1Department of Sport, Inner Mongolia Medical University, 2Department of Neurology, The Seventh Medical Center of PLA General Hospital, 3Department of Neurology, NO 984 Hospital of PLA

This study proposes an accelerometer-based method to objectively measure physical activity (PA) and leisure time physical activity (LTPA) in Chinese children accepting table tennis training in clubs.

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Environment

Resource Recycling of Red Soil to Synthesize Fe2O3/FAU-type Zeolite Composite Material for Heavy Metal Removal
Zheting Chu 1, Jiaxin Liang 1, Dazhong Yang 1, Jing Li 1, Hong Chen 1
1State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Shenzhen Key Laboratory of Interfacial Science and Engineering, Southern University of Science and Technology

This article presents a novel and convenient route to synthesize Fe2O3/faujasite (FAU)-type zeolite composite material from red soil. The detailed synthesis parameters have been finely tuned. The obtained composite material can be used for efficient heavy metal-contaminated water remediation, indicating its potential applications in environmental engineering.

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Engineering

Finite Element Modeling for the Simulation of the Quasi-Static Compression of Corrugated Tapered Tubes
Xinmei Xiang 1,2, Dehua Shao 1, Xin Zhang 3, Shaolin Zhang 4, Yijie Liu 1
1School of Civil Engineering, Guangzhou University, 2Guangdong Provincial Key Laboratory of Earthquake Engineering and Applied Technology, Guangzhou University, 3Department of Mechanics and Aerospace Engineering, Southern University of Science and Technology, 4School of Physics and Materials Science, Guangzhou University

This protocol describes the study of the quasi-static compression performance of corrugated tapered tubes using finite element simulations. The influence of the thickness gradient on the compression performance was investigated. The results show that proper thickness gradient design can change the deformation mode and significantly improve the energy absorption performance of the tubes.

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Biology

Isolation of the Brain Secretome from Ex Vivo Brain Slice Cultures
Chunyu Du 1, Huaxing Ou 2, Jianping Lu 1
1Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, 2Department of Biomedical Engineering, Southern University of Science and Technology

The brain secretome plays a pivotal role in the development and normal functioning of the central nervous system. In this article, we provide a detailed protocol for isolation of the brain secretome from ex vivo brain slice cultures.

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Engineering

Measuring the Mechanical Properties of Glass Fiber Reinforcement Polymer Composite Laminates Obtained by Different Fabrication Processes
Jin Lai 1, Xiaoyu Zhang 1, Xin Zhang 1
1Department of Mechanics and Aerospace Engineering, Southern University of Science and Technology

This paper describes a fabrication process for fiber-reinforced polymer matrix composite laminates obtained using the wet hand lay-up/vacuum bag method.

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Cancer Research

Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells
Ying Xue *1, Yunyu Mao *2, Qibin Liao 3, Chen Zhao 4, Xiaoyan Zhang 1,2,4, Jianqing Xu 1,2,4
1Institutes of Biomedical Sciences, Fudan University, 2Clinical Center of Biotherapy at Zhongshan Hospital, Fudan University, 3Department of Oncology and Bio-therapeutic Center, Shenzhen Third People's Hospital, Southern University of Science and Technology, 4Shanghai Public Health Clinical Center, Fudan University

Here we present a protocol for the generation and functional verification of hypoxia-sensitive chimeric antigen receptor (CAR)-T cells. This protocol presents the lentivirus-based generation of hypoxia-sensitive CAR-T cells and their characterization, including the validation of hypoxia-dependent CAR expression and selective cytotoxicity.

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