In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

3.1K Views

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09:06 min

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December 20th, 2021

DOI :

10.3791/63411-v

December 20th, 2021

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Wanjie Wu¹, Xuesong Li¹, Jianan Y. Qu¹^,²^,³^,⁴, Sicong He⁵

¹Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, ²State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, ³Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, ⁴Molecular Neuroscience Center, The Hong Kong University of Science and Technology, ⁵Department of Biology, School of Life Sciences, Southern University of Science and Technology

Summary

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In Vivo Imaging

Biological Tissues

Two photon Fluorescence

Stimulated Raman Scattering

Microscopy

Subcellular Resolution

Cellular Dynamics

Neurodegenerative Diseases

Spinal Cord Injury

Titanium Sapphire Laser

Optical Alignment

EOM Control

Modulation Depth

Biophysical Information

Cellular Metabolism

Chapters in this video

0:04

Introduction

0:59

Starting-Up the Lasers

2:02

Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope

4:39

Optimizing Imaging Conditions

6:36

In Vivo TPEF-SRS Imaging of Mouse Spinal Cord

8:03

Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths

8:31

Conclusion

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