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the second affiliated hospital of Guangzhou medical university

7 ARTICLES PUBLISHED IN JoVE

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Biology

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
Chuanxian Wei *1, Yan Yan *1, Xiaoli Miao 1, Renjie Jiao 1,2,3
1Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, 2The Second Affiliated Hospital of Guangzhou Medical University, 3The State Key Laboratory of Respiratory Disease, Guangzhou Medical University

Presented here are detailed methods for the dissection and lipid droplet staining of oenocytes in Drosophila larvae using BODIPY 493/503, a lipid droplet-specific fluorescent dye.

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Neuroscience

Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation
Ming Luo 1, Xiangyue Tang 1, Juehua Zhu 2, Zhihua Qiu 1, Yongjun Jiang 1
1The Second Affiliated Hospital of Guangzhou Medical University, 2Department of Neurology, The First Affiliated Hospital of Soochow University

Presented here is a protocol for establishing acute pontine infarction in a rat model via electrical stimulation with a single pulse.

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Neuroscience

Massive Pontine Hemorrhage by Dual Injection of Autologous Blood
Xiangyue Tang *1, Li Wu *1, Ming Luo 1, Zhihua Qiu 1, Yongjun Jiang 1
1The Second Affiliated Hospital of Guangzhou Medical University

We present a protocol to establish a massive pontine hemorrhage model in a rat via dual injection of autologous blood.

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Cancer Research

Application of Hemostatic Devices in Laparoscopic Hepatectomy
Shuian Xie 1, Songhang Liu 1, Youkui Gao 1, Jiancheng Tang 1, Liangqi Cao 1
1Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University

High-grade hemostatic devices are essential for laparoscopic hepatectomy. However, these devices are not generalized in basic medical organizations. Therefore, a suite of simple and easy hemostatic devices is shown in this article, which can make the laparoscopic hepatectomy easier to perform.

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Cancer Research

Application of the En Bloc Concept Combined with Anatomic Resection in Laparoscopic Hepatectomy
Haopeng Wen *1, Nan Cheng *1, Jiancheng Tang *1, Songhang Liu 1, Hongbin Zhou 1, Xinyu Li 1, Liangqi Cao 1
1Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University

Numerous studies have demonstrated the advantages of anatomic resection. Nonetheless, whether anatomic resection can increase R0 resection rates remains controversial. Consequently, the present study describes an innovative procedure involving the en bloc concept combined with anatomic resection in laparoscopic hepatectomy, which can reduce postoperative recurrence and metastasis.

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Medicine

Fluorescent Laparoscopic Central Hepatectomy for Liver Cancer Using Indocyanine Green Negative Staining
Jinxing Wei *1, Haixiong Zhang *2, Jinyi Zhong 1, Jiyan Xie 3, Weibang Yang 4, Jinxin Duan 1, Wenfeng Zhuo 1, Ang Li 1, Changzhen Shang 1, Jun Min 1
1Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 2Department of Hepatobiliary Surgery, Foshan Fosun Chancheng Hospital, 3Department of Gastrointestinal Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University, 4Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University

The present protocol describes fluorescent negative staining in laparoscopic central hepatectomy. This technique can make hepatectomy more accurate and precise.

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Medicine

Application of Laparoscopic Hepatectomy Combined with Intraoperative Microwave Ablation in Colorectal Cancer Liver Metastasis
Dawei Zhang *1, Zuxiao Chen *2, Lei Zhang 1, Xiangjun Qian 1, Xiaoming Huang 1, Zheyu Zheng 1, Weidong Pan 1
1Department of Pancreatic Hepatobiliary Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, 2Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University

This protocol describes the laparoscopic resection of colorectal cancer liver metastases combined with ultrasound-guided microwave ablation. This technique can safely, effectively, and accurately treat refractory liver metastases <3 cm, reduce postoperative complications, and accelerate the postoperative rehabilitation of patients.

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