University of Montana

7 ARTICLES PUBLISHED IN JoVE

Environment

Quantifying Corticolous Arthropods Using Sticky Traps

Michael W. Eichholz^*1, Elise C. Zarri^*2, Kevin P. Sierzega^*1

¹Cooperative Wildlife Research Laboratory, Center for Ecology, Department of Zoology, Southern Illinois University, ²Montana Cooperative Research Unit, University of Montana

We describe a semi-quantitative approach of measuring characteristics of corticolous (bark-dwelling) arthropod communities. We placed commercially manufactured sticky traps on tree boles to estimate abundance, total length (a surrogate to biomass), richness, and Shannon diversity for comparison among tree species.

Developmental Biology

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Nicholas J. Day¹, Xiaobo Wang¹, Ekaterina Voronina¹

¹Division of Biological Sciences, University of Montana

This protocol demonstrates use of the proximity ligation assay to probe for protein-protein interactions in situ in the C. elegans germline.

Biochemistry

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Matthew J. Gaffrey¹, Nicholas J. Day¹, Xiaolu Li², Wei-Jun Qian¹

¹Integrative Omics, Biological Sciences Division, Pacific Northwest National Laboratory, ²Bioproducts Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University

Protein thiol oxidation has significant implications under normal physiological and pathophysiological conditions. We describe the details of a quantitative redox proteomics method, which utilizes resin-assisted capture, isobaric labeling, and mass spectrometry, enabling site-specific identification and quantification of reversibly oxidized cysteine residues of proteins.

Medicine

Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter

Pengmei Guo^*1, Wenqiao An^*2, Ying Guo¹, Zhihao Sun², Xiaobo Wang^1,3, Sanyin Zhang^1,3

¹Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ²School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, ³Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine

The present protocol describes the wire myograph technique for measuring vascular reactivity of the rat coronary artery.

Neuroscience

Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System

Xiaobo Wang^1,2, Na Xie³, Yi Zhang⁴, Xianli Meng^1,2,3, Ya Hou³, Sanyin Zhang^1,2

¹Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ²Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, ³School of Pharmacy, Chengdu University of Traditional Chinese Medicine, ⁴School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine

The protocol here provides a detailed real-time dynamic sampling of extracellular fluid from the hippocampus of awake rats using a microdialysis system.

Medicine

Dynamic Continuous Blood Extraction from Rat Heart via Noninvasive Microdialysis Technique

Ya Hou¹, Jinrong Bai^1,2, Yi Zhang³, Xianli Meng^1,2,4, Sanyin Zhang⁴, Xiaobo Wang²

¹School of Pharmacy, Chengdu University of Traditional Chinese Medicine, ²Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, ⁴Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine

The present protocol describes a simple and efficient method for the real-time and dynamic collection of rat heart blood using the microdialysis technique.

Medicine

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Hong Jiang^*1, Yating Zhang^*1, Ya Hou¹, Lijun Li², Sanyin Zhang^3,4, Yi Zhang⁵, Xianli Meng^1,3,4, Xiaobo Wang⁴

¹State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, ²Chengdu Techman Instrument Co., Ltd., ³Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ⁴Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, ⁵School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine

The present protocol describes an efficient method for the real-time and dynamic acquisition of voltage-gated potassium (Kv) channel currents in H9c2 cardiomyocytes using the whole-cell patch-clamp technique.