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University of California, San Francisco

11 ARTICLES PUBLISHED IN JoVE

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Biology

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development
Diane Hu 1, Ralph S. Marcucio 1
1Department of Orthopaedic Surgery, University of California San Francisco

This article describes a tissue transplantation technique that was designed to test the signaling and patterning properties of surface cephalic ectoderm during craniofacial development.

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Neuroscience

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
Dimitrios Davalos 1, Katerina Akassoglou 1,2
1Gladstone Institute of Neurological Disease, University of California, San Francisco , 2Department of Neurology, University of California, San Francisco

A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.

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Medicine

Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects
Yan-yiu Yu 1, Chelsea Bahney 1, Diane Hu 1, Ralph S. Marcucio 1, Theodore Miclau, III 1
1Department of Orthopaedic Surgery, University of California, San Francisco

This article describes a method for stabilizing long bone fractures that is based on the application of modified Ilizarov external fixators 1-3. After application of the fixators and creation of the bone injury, healing can be assessed, distraction osteogenesis can be performed, or non-union or critical sized defect can be created and used to study therapeutic interventions.

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Biology

Synthesis of an In vivo MRI-detectable Apoptosis Probe
Justin Lam 1, Paul C. Simpson 2,3, Phillip C. Yang 1, Rajesh Dash 1
1Division of Cardiovascular Medicine, Department of Medicine, Stanford University Medical Center, 2Division of Cardiology, Department of Medicine, University of California, San Francisco , 3San Francisco VAMC

Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.

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Medicine

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment
Moo Rim Kang *1, Glen Yang *1, Klaus Charisse 2, Hila Epstein-Barash 2, Muthiah Manoharan 2, Long-Cheng Li 1
1Department of Urology and Helen Diller Comprehensive Cancer Center, University of California, San Francisco , 2Alnylam Pharmaceuticals, Inc.

Establishing an orthotopic bladder tumor model to evaluate antitumor effects of intravesically delivered saRNA and monitoring tumor growth by ultrasound and bioluminescent imaging.

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Medicine

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
David D. Lo *1,2, Jeong S. Hyun *1,3, Michael T. Chung 1, Daniel T. Montoro 1, Andrew Zimmermann 1, Monica M. Grova 1,4, Min Lee 5, Derrick C. Wan 1, Michael T. Longaker 1
1Department of Surgery, Stanford University , 2Department of Surgery, Duke University , 3Department of Surgery, Saint Joseph Mercy Hospital, 4School of Medicine, University of California, San Francisco , 5School of Dentistry, University of California, Los Angeles

This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.

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Biology

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
Carolina L. Haass-Koffler 1,2, Mohammad Naeemuddin 1, Selena E. Bartlett 1,3
1Medications Development, Ernest Gallo Clinic and Research Center, University of California, San Francisco , 2Clinical Pharmacology and Experimental Therapeutics, University of California, San Francisco , 3Translational Research Institute and the Institute for Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia

We developed a software platform that utilizes Imaris Neuroscience, ImarisXT and MATLAB to measure the changes in morphology of an undefined shape taken from three-dimensional confocal fluorescence of single cells. This novel approach can be used to quantify changes in cell shape following receptor activation and therefore represents a possible additional tool for drug discovery.

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Neuroscience

Forebrain Electrophysiological Recording in Larval Zebrafish
Scott C. Baraban 1
1Epilepsy Research Laboratory, Department of Neurological Surgery, University of California, San Francisco

A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.

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Biology

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
Ulrike Liisberg Aune 1,2,3, Lauren Ruiz 1, Shingo Kajimura 1
1UCSF Diabetes Center and Department of Cell and Tissue Biology, University of California, San Francisco , 2Department of Biology, University of Copenhagen, Denmark, 3National Institute of Nutrition and Seafood Research, Bergen, Norway

Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.

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Bioengineering

Magnetically-Assisted Remote Controlled Microcatheter Tip Deflection under Magnetic Resonance Imaging
Steven W. Hetts 1, Maythem Saeed 1, Alastair Martin 1, Prasheel Lillaney 1, Aaron Losey 2, Erin Jeannie Yee 1, Ryan Sincic 3, Loi Do 1, Lee Evans 1, Vincent Malba 1, Anthony F. Bernhardt 1, Mark W. Wilson 1, Anand Patel 1, Ronald L. Arenson 4, Curtis Caton 5, Daniel L. Cooke 1
1Department of Radiology and Biomedical Imaging, University of California, San Francisco , 2School of Medicine, University of California, San Francisco , 3Department of Radiology and Biomedical Imaging, UCSF Medical Center, 4University of California, San Francisco , 5Hansen Medical, Mountain View, CA

Current applied to an endovascular microcatheter with microcoil tip made by laser lathe lithography can achieve controllable deflections under magnetic resonance (MR) guidance, which may improve speed and efficacy of navigation of vasculature during various endovascular procedures.

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Medicine

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis
Rishu Gupta 1,2, Maya Debbaneh 2,3, Daniel Butler 2,4, Monica Huynh 2,5, Ethan Levin 2, Argentina Leon 2, John Koo 2, Wilson Liao 2
1Keck School of Medicine, University of Southern California, 2Psoriasis and Skin Treatment Center Dermatology, University of California, San Francisco , 3University of California Irvine School of Medicine, 4University of Arizona College of Medicine, 5Chicago College of Osteopathic Medicine

Psoriasis is a chronic, immune-mediated inflammatory skin disease. The Goeckerman regimen, formulated for the treatment of psoriasis, consists of exposure to ultraviolet B (UVB) light and application of crude coal tar (CCT). The following protocol is for the administration of Goeckerman therapy for the treatment of moderate-to-severe psoriasis.

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