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In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

DOI :

10.3791/2760-v

January 5th, 2012

January 5th, 2012

23,210 Views

1Gladstone Institute of Neurological Disease, University of California, San Francisco , 2Department of Neurology, University of California, San Francisco

A minimally invasive protocol to stabilize the mouse spinal column and perform repetitive in vivo spinal cord imaging using two-photon microscopy is described. This method combines a spinal stabilization device and an anesthetic regimen to minimize respiratory-induced movements and produce raw imaging data that require no alignment or other post-processing.

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