Accedi

Institut Pasteur

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Stéphanie Beaucourt¹, Antonio V. Bordería¹, Lark L. Coffey¹, Nina F. Gnädig¹, Marta Sanz-Ramos¹, Yasnee Beeharry¹, Marco Vignuzzi¹

¹Viral Populations and Pathogenesis lab and CNRS 3015, Institut Pasteur

The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.

Immunology and Infection

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Mandy Muller^*1,2, Patricia Cassonnet^*1, Michel Favre¹, Yves Jacob^1,3, Caroline Demeret¹

¹Unité de Génétique, Papillomavirus et Cancer Humain (GPCH), Institut Pasteur , ²Cellule Pasteur, Université Sorbonne Paris Cité, ³Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology, Dana Farber Cancer Institute

This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.