Institut Pasteur
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Stéphanie Beaucourt 1, Antonio V. Bordería 1, Lark L. Coffey 1, Nina F. Gnädig 1, Marta Sanz-Ramos 1, Yasnee Beeharry 1, Marco Vignuzzi 1
1Viral Populations and Pathogenesis lab and CNRS 3015, Institut Pasteur
The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.
Immunology and Infection
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Mandy Muller *1,2, Patricia Cassonnet *1, Michel Favre 1, Yves Jacob 1,3, Caroline Demeret 1
1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH), Institut Pasteur , 2Cellule Pasteur, Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology, Dana Farber Cancer Institute
This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.
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