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Eindhoven University of Technology

19 ARTICLES PUBLISHED IN JoVE

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Medicine

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice
Michael T. Kuhlmann 1, Simon Cuhlmann 2,3, Irmgard Hoppe 1, Rob Krams 3, Paul C. Evans 2, Gustav J. Strijkers 4, Klaas Nicolay 4, Sven Hermann 1, Michael Schäfers 1
1European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, 2British Heart Foundation Cardiovascular Sciences Unit, Imperial College London , 3Department of Bioengineering, Imperial College London , 4Biomedical Engineering, Eindhoven University of Technology

The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes.

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Engineering

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
Pol Besenius 1, Isja de Feijter 2, Nico A.J.M. Sommerdijk 3, Paul H.H. Bomans 3, Anja R. A. Palmans 2
1Organic Chemistry Institute and CeNTech, Westfälische Wilhelms-Universität Münster, 2Laboratory of Macromolecular and Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, 3Laboratory of Materials and Interface Chemistry and Soft Matter Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology

The goal of this experiment is to determine and control the size, shape and stability of self-assembled discotic amphiphiles in water. For aqueous based supramolecular polymers such level of control is very difficult. We apply a strategy using both repulsive and attractive interactions. The experimental techniques applied to characterize this system are broadly applicable.

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Bioengineering

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
Daisy W. J. van der Schaft 1, Ariane C. C. van Spreeuwel 1, Kristel J. M. Boonen 1, Marloes L. P. Langelaan 1, Carlijn V. C. Bouten 1, Frank P. T. Baaijens 1
1Department of Biomedical Engineering, Soft Tissue Biomechanics and Engineering, Eindhoven University of Technology, The Netherlands

Engineered muscle tissue has great potential in regenerative medicine, as disease model and also as an alternative source for meat. Here we describe the engineering of a muscle construct, in this case from mouse myoblast progenitor cells, and the stimulation by electrical pulses.

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Bioengineering

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
Nicky de Jonge 1, Frank P. T. Baaijens 1, Carlijn V. C. Bouten 1
1Department of Biomedical Engineering, Eindhoven University of Technology

This model system starts from a myofibroblast-populated fibrin gel that can be used to study endogenous collagen (re)organization real-time in a nondestructive manner. The model system is very tunable, as it can be used with different cell sources, medium additives, and can be adapted easily to specific needs.

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Bioengineering

An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart
A. C. H. Pape *1, Maarten H. Bakker *1, Cheyenne C. S. Tseng 2, Maartje M. C. Bastings 1, Stefan Koudstaal 2, Pierfrancesco Agostoni 2, Steven A. J. Chamuleau 2, Patricia Y. W. Dankers 1
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, 2Department of Cardiology, Division Heart and Lungs, Interuniversity Cardiology Institute of the Netherlands (ICIN), University Medical Center Utrecht

Supramolecular hydrogelators based on ureido-pyrimidinones allow full control over the macroscopic gel properties and the sol–gel switching behavior using pH. Here, we present a protocol for formulating and injecting such a supramolecular hydrogelator via a catheter delivery system for local delivery directly in relevant areas in the pig heart.

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Engineering

Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model
İlker Doğan 1, Mauritius C. M. van de Sanden 1,2
1Department of Applied Physics, Eindhoven University of Technology, 2Dutch Institute for Fundamental Energy Research

We demonstrate how to determine the size distribution of semiconductor nanocrystals in a quantitative manner using Raman spectroscopy employing an analytically defined multi-particle phonon confinement model. Results obtained are in excellent agreement with the other size analysis techniques like transmission electron microscopy and photoluminescence spectroscopy.

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Bioengineering

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
Sandra Van de Wiel 1, Maarten Merkx 2,3, Stan Van de Graaf 1
1Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, 2Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), 3Department of Biomedical Engineering, Eindhoven University of Technology

We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.

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Education

Synthesis and Characterization of Supramolecular Colloids
Neus Vilanova 1,4, Isja De Feijter 1,2,4, Ilja K. Voets 1,3,4
1Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, 2Laboratory of Chemical Biology, Eindhoven University of Technology, 3Laboratory of Physical Chemistry, Eindhoven University of Technology, 4Institute for Complex Molecular Systems, Eindhoven University of Technology

A protocol for the synthesis and characterization of colloids coated with supramolecular moieties is described. These supramolecular colloids undergo self-assembly upon the activation of the hydrogen-bonds between the surface-anchored molecules by UV-light.

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JoVE Core

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry
Dirk van den Bekerom 1, Niek den Harder 1, Teofil Minea 1, Nicola Gatti 1,2, Jose Palomares Linares 1, Waldo Bongers 1, Richard van de Sanden 1,3, Gerard van Rooij 1,3
1Dutch Institute for Fundamental Energy Research, 2University of Trento, 3Eindhoven University of Technology

This article describes a flowing microwave reactor that is used to drive efficient non-equilibrium chemistry for the application of conversion/activation of stable molecules such as CO2, N2 and CH4. The goal of the procedure described here is to measure the in situ gas temperature and gas conversion.

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Bioengineering

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
Marinke W. van der Helm 1, Mathieu Odijk 1, Jean-Philippe Frimat 1,2, Andries D. van der Meer 3, Jan C.T. Eijkel 1, Albert van den Berg 1, Loes I. Segerink 1
1BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics, University of Twente, 2Microsystems, Eindhoven University of Technology, 3Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente

This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.

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Bioengineering

Anatomically Realistic Neonatal Heart Model for Use in Neonatal Patient Simulators
Mark Thielen 1, Frank Delbressine 1, Sidarto Bambang Oetomo 1,2, Loe Feijs 1
1Department of Industrial Design, Eindhoven University of Technology, 2Department of Neonatology, Máxima Medisch Centrum Veldhoven

This protocol describes a procedure for creating functional artificial neonatal heart models by utilizing a combination of magnetic resonance imaging, 3D printing, and injection molding. The purpose of these models is for integration into the next generation of neonatal patient simulators and as a tool for physiological and anatomical studies.

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Engineering

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
Nidhi Sinha *1, Nikita Subedi *1, Florian Wimmers *2, Melf Soennichsen 1, Jurjen Tel 1
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering, Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center, Stanford University

This article presents a protocol for seeding scarce population of cells using pipette-tips to droplet microfluidic devices in order to provide higher encapsulation efficiency of cells in droplets.

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Bioengineering

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Ardjan J. van der Linden 1, Maaruthy Yelleswarapu 2, Pascal A. Pieters 1, Zoe Swank 3, Wilhelm T. S. Huck 2, Sebastian J. Maerkl 3, Tom F. A. de Greef 1,2
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.

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Engineering

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch
Suzanne E. Koch 1,2, Eline E. van Haaften 1,2, Tamar B. Wissing 1,2, Lizzy A. B. Cuypers 1, Jurgen A. Bulsink 1, Carlijn V. C. Bouten 1,2, Nicholas A. Kurniawan *1,2, Anthal I. P. M. Smits *1,2
1Department of Biomedical Engineering, Eindhoven University of Technology, 2Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology

The goal of this protocol is to execute a dynamic co-culture of human macrophages and myofibroblasts in tubular electrospun scaffolds to investigate material-driven tissue regeneration, using a bioreactor which enables the decoupling of shear stress and cyclic stretch.

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Bioengineering

Quantification of Mouse Heart Left Ventricular Function, Myocardial Strain, and Hemodynamic Forces by Cardiovascular Magnetic Resonance Imaging
Mariah R. R. Daal 1, Gustav J. Strijkers 1,2, Claudia Calcagno 2, Ruslan R. Garipov 3, Rob C. I. Wüst 1,4, David Hautemann 5, Bram F. Coolen 1
1Department of Biomedical Engineering & Physics, Amsterdam University Medical Centers, Amsterdam Cardiovascular Sciences, University of Amsterdam, 2BioMedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, 3MR Solutions Ltd., 4Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioral and Movement Sciences, Vrije Universiteit Amsterdam, 5Medis medical imaging systems B.V.

This study describes a comprehensive cardiovascular magnetic resonance imaging (CMR) protocol to quantify the left ventricular functional parameters of the mouse heart. The protocol describes the acquisition, post-processing, and analysis of the CMR images as well as assessment of different cardiac functional parameters.

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Bioengineering

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
Cas van der Putten 1,2, Mirko D’Urso 1,2, Maaike Bril 1,2, Thomas E. Woud 1,2, Carlijn V. C. Bouten 1,2, Nicholas A. Kurniawan 1,2
1Department of Biomedical Engineering, Eindhoven University of Technology, 2Institute for Complex Molecular Systems, Eindhoven University of Technology

Traditionally, cell culture is performed on planar substrates that poorly mimic the natural environment of cells in vivo. Here we describe a method to produce cell culture substrates with physiologically relevant curved geometries and micropatterned extracellular proteins, allowing systematic investigations into cellular sensing of these extracellular cues.

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Bioengineering

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue
Hanneke Crielaard 1, Su Guvenir Torun 1, Tamar B. Wissing 1,2, Pablo de Miguel Muñoz 1,3, Gert-Jan Kremers 4, Frank J. H. Gijsen 1,3, Kim Van Der Heiden 1,2, Ali C. Akyildiz 1,3
1Department of Biomedical Engineering, Erasmus Medical Center, 2Department of Biomedical Engineering, Eindhoven University of Technology, 3Department of Biomechanical Engineering, Delft University of Technology, 4Erasmus Optical Imaging Center, Erasmus Medical Center

We have developed a mechano-imaging pipeline to study the heterogeneous structural and mechanical atherosclerotic plaque properties. This pipeline enables correlation of the local predominant angle and dispersion of collagen fiber orientation, the rupture behavior, and the strain fingerprints of the fibrous plaque tissue.

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Engineering

Preparation of Free-Surface Hyperbolic Water Vortices
Roman Klymenko 1,5, Harmen Nanninga 2, Esther de Kroon 1, Luewton L. F. Agostinho 1,2, Elmar C. Fuchs 1,3, Jakob Woisetschläger 4, Wilfred F. L. M. Hoeben 5
1Wetsus - Centre of Excellence for Sustainable Water Technology, 2Water Technology Research Group, NHL Stenden University of Applied Sciences, 3Optical Sciences Group, Faculty of Science and Technology (TNW), University of Twente, 4Institute for Thermal Turbomachinery and Machine Dynamics, Graz University of Technology, 5Department of Electrical Engineering, Electrical Energy Systems group, Eindhoven University of Technology

This paper describes how three different water vortex regimes in a hyperbolic Schauberger funnel can be created, their most important characteristics, and how associated parameters such as the oxygen transfer rates can be calculated.

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Bioengineering

Bilayer Microfluidic Device for Combinatorial Plug Production
Maaruthy Yelleswarapu 1, Sofia Spinthaki 1, Tom F. A. de Greef 1, Federica Eduati 1
1Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology

The fabrication of a polydimethylsiloxane (PDMS)-based bilayer device for the production of combinatorial libraries in water-in-oil emulsions (plugs) is presented here. The necessary hardware and software required to automate plug production are detailed in the protocol, and the production of a quantitative library of fluorescent plugs is also demonstrated.

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