Accedi

The University of Kansas School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Biology

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

Randal Halfmann^1,2,3, Susan Lindquist^1,2,3

¹Whitehead Institute for Biomedical Research, ²Department of Biology, MIT - Massachusetts Institute of Technology, ³Howard Hughes Medical Institute

SDD-AGE is a useful technique for the detection and characterization of amyloid-like polymers in cells. Here we demonstrate an adaptation that makes this technique amenable to large-scale applications.

Neuroscience

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor

Limei Ma¹, Sachiko Haga-Yamanaka¹, Qingfeng Elden Yu¹, Qiang Qiu¹, SangSeong Kim¹, C. Ron Yu^1,2

¹Stowers Institute for Medical Research, ²Department of Anatomy and Cell Biology, The University of Kansas School of Medicine

The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli.

Biology

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Shriram Venkatesan^*1, Tejbir S. Kandola^*1, Alejandro Rodríguez-Gama¹, Andrew Box¹, Randal Halfmann^1,2

¹Stowers Institute for Medical Research, ²Department of Molecular and Integrative Physiology, The University of Kansas School of Medicine

This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.