Accedi

Max Planck Institute for Psycholinguistics

2 ARTICLES PUBLISHED IN JoVE

Biology

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Pelagia Deriziotis^*1, Sarah A. Graham^*1, Sara B. Estruch¹, Simon E. Fisher^1,2

¹Language and Genetics Department, Max Planck Institute for Psycholinguistics, ²Donders Institute for Brain, Cognition and Behaviour

Interactions between proteins are fundamental to all cellular processes. Using Bioluminescence Resonance Energy Transfer, the interaction between a pair of proteins can be monitored in live cells and in real time. Furthermore, the effects of potentially pathogenic mutations can be assessed.

Biology

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Laurel R. Yohe^*1,2, Paolo Devanna^*3, Kalina T.J. Davies⁴, Joshua H.T. Potter⁴, Stephen J. Rossiter⁴, Emma C. Teeling⁵, Sonja C. Vernes^*3,6, Liliana M. Dávalos^*2,7

¹Department of Geology & Geophysics, Yale University, ²Department of Ecology & Evolution, Stony Brook University, ³Neurogenetics of Vocal Communication, Max Planck Institute for Psycholinguistics, ⁴School of Biological and Chemical Sciences, Queen Mary University of London, ⁵School of Biology & Environmental Science, University College Dublin, ⁶Donders Institute for Brain, Cognition and Behavior, ⁷Consortium for Inter-Disciplinary Environmental Research, Stony Brook University

This is a protocol for the optimal tissue preparation for genomic, transcriptomic, and proteomic analyses of bats caught in the wild. It includes protocols for bat capture and dissection, tissue preservation, and cell culturing of bat tissue.