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University of California, Davis

6 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Microfluidic Mixers for Studying Protein Folding
Steven A. Waldauer 1, Ling Wu 1, Shuhuai Yao 2, Olgica Bakajin 3, Lisa J. Lapidus 1
1Department of Physics and Astronomy, Michigan State University, 2Department of Mechanical Engineering, Hong Kong University of Science and Technology, 3Center for Biophotonics, University of California, Davis

In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).

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Bioengineering

On-Chip Endothelial Inflammatory Phenotyping
J. Sherrod DeVerse *1, Keith A. Bailey *1, Greg A. Foster 1, Vaishali Mittal 1, Stuart M. Altman 1, Scott I. Simon 1, Anthony G. Passerini 1
1Department of Biomedical Engineering, University of California, Davis

Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.

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JoVE Journal

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
Chun A. Changou 1,2, Deanna L. Wolfson 2, Balpreet Singh Ahluwalia 2,3, Richard J. Bold 4,5, Hsing-Jien Kung 5,6, Frank Y.S. Chuang 1,2,5
1Department of Biochemistry and Molecular Medicine, University of California, Davis , 2NSF Center for Biophotonics Science & Technology, University of California, Davis , 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis , 5UC Davis Comprehensive Cancer Center, University of California, Davis , 6Department of Biological Chemistry, University of California, Davis

Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.

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Bioengineering

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies
Pallavi Daggumati 1, Ozge Kurtulus 2, Christopher Abbott Reece Chapman 3, Damla Dimlioglu 1, Erkin Seker 1
1Department of Electrical and Computer Engineering, University of California, Davis , 2Department of Chemical Engineering and Materials Science, University of California, Davis , 3Department of Biomedical Engineering, University of California, Davis

We report on techniques to micropattern nanoporous gold thin films via stencil printing and photolithography, as well as methods to culture cells on the microfabricated patterns. In addition, we describe image analysis methods to characterize morphology of the material and the cultured cells using scanning electron and fluorescence microscopy techniques.

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Biology

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
Wan-Shan Yang 1, Mel Campbell 2, Hsing-Jien Kung 2,3,4,5, Pei-Ching Chang 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

Unlike ubiquitin ligases, few E3 SUMO ligases have been identified. This modified in vitro SUMOylation protocol is able to identify novel SUMO E3 ligases by an in vitro reconstitution assay.

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Immunology and Infection

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection
Leif S. Anderson 1, Mack B. Reynolds 1, Kathryn R. Rivara 1, Lloyd S. Miller 2, Scott I. Simon 1
1Department of Biomedical Engineering, University of California Davis, 2Department of Dermatology, Johns Hopkins University School of Medicine

An approach is described for real-time detection of the innate immune response to cutaneous wounding and Staphylococcus aureus infection of mice. By comparing LysM-EGFP mice (which possess fluorescent neutrophils) with a LysM-EGFP crossbred immunodeficient mouse strain, we advance our understanding of infection and the development of approaches to combat infection.

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