Accedi

RIKEN Center for Integrative Medical Sciences

2 ARTICLES PUBLISHED IN JoVE

Genetics

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Hazuki Takahashi^*1, Harshita Sharma^*1, Piero Carninci^1,2

¹Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, ²TransSINE Technologies Co. Ltd.

SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.

Neuroscience

Purification of the Dendritic Filopodia-rich Fraction

Yutaka Furutani^1,2, Yoshihiro Yoshihara³

¹Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, ²Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, ³Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.