Accedi

Korea Research Institute of Bioscience and Bioengineering (KRIBB)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Jae H. Lee^1,2, Yong II Cho⁵, Sung S. Choi⁴, Hae-Won Kim^1,2, Churl K. Min³, Sang J. Lee⁵

¹Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, ²Insititute of Tissue Regeneration Engineering, Dankook University, ³Department of Biological Sciences, Ajou University, ⁴Department of Pharmacy, Sahmyook University, ⁵Department of Animal Biotechnology, Sahmyook University

The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.

JoVE Journal

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Haeyoung Jeong^*1, Sang J. Lee^*2, Pil Kim³

¹Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ²Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), ³Department of Biotechnology, The Catholic University of Korea

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.