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Korea Research Institute of Bioscience and Bioengineering (KRIBB)

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Jae H. Lee 1,2, Yong II Cho 5, Sung S. Choi 4, Hae-Won Kim 1,2, Churl K. Min 3, Sang J. Lee 5
1Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, 2Insititute of Tissue Regeneration Engineering, Dankook University, 3Department of Biological Sciences, Ajou University, 4Department of Pharmacy, Sahmyook University, 5Department of Animal Biotechnology, Sahmyook University

The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.

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JoVE Journal

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Haeyoung Jeong *1, Sang J. Lee *2, Pil Kim 3
1Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), 3Department of Biotechnology, The Catholic University of Korea

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.

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