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Université de Lyon, ENS de Lyon, UCBL, INRA, CNRS

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues

Simone Bovio^1,2, Yuchen Long², Françoise Monéger²

¹SFR Biosciences, Université de Lyon, ²Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCBL, INRA, CNRS

Here, we present atomic force microscopy (AFM), operated as a nano- and micro-indentation tool on cells and tissues. The instrument allows the simultaneous acquisition of 3D surface topography of the sample and its mechanical properties, including cell wall Young's modulus as well as turgor pressure.