Accedi

Alcon Vision, LLC

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Development of an In Vitro Ocular Platform to Test Contact Lenses

Chau-Minh Phan¹, Hendrik Walther¹, Huayi Gao², Jordan Rossy¹, Lakshman N. Subbaraman¹, Lyndon Jones¹

¹School of Optometry and Vision Science, University of Waterloo, ²Medella Health

Current in vitro models for evaluating contact lenses (CLs) and other eye-related applications are severely limited. The presented ocular platform simulates physiological tear flow, tear volume, air exposure and mechanical wear. This system is highly versatile and can be applied to various in vitro analyses with CLs.

Medicine

Impression Cytology of the Lid Wiper Area

Alex Muntz¹, Kevin van Doorn¹, Lakshman N. Subbaraman¹, Lyndon W. Jones¹

¹Centre for Contact Lens Research, University of Waterloo

We collected, stained and imaged cells from the conjunctiva of the inner eyelid margin of human subjects. By characterizing cell morphology and metabolic activity, this method may further our understanding of dry eye and the role that friction between the ocular surfaces may play in perceiving ocular discomfort.

Medicine

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Rekha Rangarajan¹, Howard A. Ketelson¹, Richard Do², David J. McCanna², Adeline Suko², Daryl Enstone², Lakshman N. Subbaraman¹, Jaya Dantam², Lyndon W. Jones²

¹Alcon Vision, LLC, ²Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science, University of Waterloo

The purpose of this protocol is to evaluate if different artificial tear formulations can protect human corneal epithelial cells from desiccation using an in vitro model. After exposure to artificial tear formulations and desiccation, human corneal epithelial cells are assessed for metabolic activity.