Accedi

Guangdong Academy of Medical Sciences

3 ARTICLES PUBLISHED IN JoVE

Genetics

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Wilson Pak-Kin Lou^*1,2, Liqun Wang^*3, Cheng Long¹, Lei Liu³, Ji-Feng Fei³

¹School of Life Sciences, South China Normal University, ²The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR), South China Normal University

Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.

Medicine

Avoiding Ischemia Reperfusion Injury in Liver Transplantation

Yixi Zhang^*1,2,3, Changjun Huang^*1,2,3, Weiqiang Ju^*1,2,3, Qiang Zhao^1,2,3, Maogen Chen^1,2,3, Linhe Wang^1,2,3, Dongping Wang^1,2,3, Linwei Wu^1,2,3, Zebin Zhu⁴, Shanzhou Huang⁵, Zhiheng Zhang^1,2,3, Caihui Zhu^1,2,3, Rongxing Xie^1,2,3, Yinghua Chen^1,2,3, Anbin Hu^1,2,3, Xiaofeng Zhu^1,2,3, Zhiyong Guo^1,2,3, Xiaoshun He^1,2,3

¹Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, ²Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, ³Guangdong Provincial International Cooperation Base of Science and Technology, ⁴Organ Transplant Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, ⁵Department of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences

Presented here is a protocol to provide a step-by-step ischemia-free liver transplantation protocol under ex situ normothermic machine perfusion (37 °C) of human livers from donors to recipients.

Biology

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Shaohui Zheng^1,2, Fang Liang³, Yu Zhang^1,2, Ji-Feng Fei⁴, Wei Qin^1,2, Yanmei Liu^1,2

¹Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, ²Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, ³Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, ⁴Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences

This protocol describes how to perform efficient adenine base editing without PAM limitation to construct a precise zebrafish disease model using zSpRY-ABE8e.