Decellularization of the Murine Cardiopulmonary ComplexAlejandro E. Mayorca-Guiliani 1, Maria Rafaeva 1, Oliver Willacy 1, Chris D. Madsen 2, Raphael Reuten 1, Janine T. Erler 1
1Biotech Research and Innovation Centre (BRIC), University of Copenhagen (UCPH), 2Department of Laboratory Medicine, Division of Translational Cancer Research, Lund University
This protocol aims to decellularize the heart and lungs of mice. The resulting extracellular matrix (ECM) scaffolds can be immunostained and imaged to map the location and topology of their components.