Il finanziamento fornito dal borse Ribble G. nel Dipartimento di Biologia, il Kentucky Giovani Ricercatori universitari e Istruzione-eureka! Ufficio (Univ. di KY).

<ol>
	<li>Ashton, K., Wagoner, A. P., Carrillo, R., Gibson, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+trait+loci+for+the+monoamine-related+traits+heart+rate+and+headless+behavior+in+Drosophila+melanogaster.">Quantitative trait loci for the monoamine-related traits heart rate and headless behavior in Drosophila melanogaster.</a> Genetics. 157, 283-294 (2001).</li><li>Azpiazu, N., Frasch, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tinman+and+Bagpipe:+Two+homeo+box+genes+that+determine+cell+fates+in+the+dorsal+mesoderm+of+Drosophila.">Tinman and Bagpipe: Two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.</a> Genes &#38; Development. 7, 1325-1340 (1993).</li><li>Badre, N. H., Martin, M. E., Cooper, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+physiological+and+behavioral+effects+of+carbon+dioxide+on+Drosophila+melanogaster+larvae.">The physiological and behavioral effects of carbon dioxide on Drosophila melanogaster larvae.</a> Comp Biochem Physiol A Mol Integr Physiol. 140, 363-376 (2005).</li><li>Baker, J. D., McNabb, S. L., Truman, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+hormonal+coordination+of+behavior+and+physiology+at+adult+ecdysis+in+Drosophila+melanogaster.">The hormonal coordination of behavior and physiology at adult ecdysis in Drosophila melanogaster.</a> J. Exp. Biol. 202, 3037-3048 (1999).</li><li>Bier, E., Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drosophila,+an+emerging+model+for+cardiac+disease.">Drosophila, an emerging model for cardiac disease.</a> Gene. 342, 1-11 (2004).</li><li>Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+gene+tinman+is+required+for+specification+of+the+heart+and+visceral+muscles+in+Drosophila.">The gene tinman is required for specification of the heart and visceral muscles in Drosophila.</a> Development. 118, 719-729 (1993).</li><li>Bodmer, R., Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+homeobox-containing+gene,+msh-2,+is+transiently+expressed+early+during+mesoderm+formation+of+Drosophila.">A new homeobox-containing gene, msh-2, is transiently expressed early during mesoderm formation of Drosophila.</a> Development. 110, 661-669 (1990).</li><li>Cooper, A. S., Cooper, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+activity+of+Drosophila+larvae:+Impedance+&#38;+video+microscopy+measures.">Monitoring activity of Drosophila larvae: Impedance &#38; video microscopy measures.</a> Drosophila Information Service. 87, 85-87 (2004).</li><li>Dasari, S., Cooper, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+influence+of+serotonin+on+the+larval+heart+of+Drosophila+melanogaster.">Direct influence of serotonin on the larval heart of Drosophila melanogaster.</a> J. Comp. Physiol. B. 176, 349-357 (2006).</li><li>Dasari, S., Viele, K., Turner, A. C., Cooper, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+p-CPA+and+MDMA+on+physiology,+development+and+behavior+in+Drosophila+melanogaster.">Influence of p-CPA and MDMA on physiology, development and behavior in Drosophila melanogaster.</a> European J. Neurosci. 26, 424-438 (2007).</li><li>Dowse, H., Ringo, J., Power, J., Johnson, E., Kinney, K., White, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+congenital+heart+defect+in+Drosophila+caused+by+an+action-potential+mutation.">A congenital heart defect in Drosophila caused by an action-potential mutation.</a> J. Neurogenetics. 10, 153-168 (1995).</li><li>Dulcis, D., Levine, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glutamatergic+innervation+of+the+heart+initiates+retrograde+contractions+in+adult+Drosophila+melanogaster.">Glutamatergic innervation of the heart initiates retrograde contractions in adult Drosophila melanogaster.</a> J. Neurosci. 25, 271-280 (2005).</li><li>Feng, Y., Ueda, A., Wu, C. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+minimal+hemolymph-like+solution,+HL3.1,+for+physiological+recordings+at+the+neuromuscular+junctions+of+normal+and+mutant+Drosophila+larvae.">A modified minimal hemolymph-like solution, HL3.1, for physiological recordings at the neuromuscular junctions of normal and mutant Drosophila larvae.</a> J. Neurogenetics. 18, 377-402 (2004).</li><li>Ganetzky, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+analysis+of+ion+channel+dysfunction+in+Drosophila.">Genetic analysis of ion channel dysfunction in Drosophila.</a> Kidney International. 3, 766-771 (2000).</li><li>GG, G. u. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pharmacological+analysis+of+heartbeat+in.">Pharmacological analysis of heartbeat in.</a> Drosophila. J. Neurobiol. 28, 269-280 (1995).</li><li>He, B., Adler, P. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+mechanisms+in+the+development+of+the+Drosophila+arista.">Cellular mechanisms in the development of the Drosophila arista.</a> Mechanisms of Development. 104, 69-78 (2001).</li><li>Johnson, E., Ringo, J., Dowse, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+Drosophila+heartbeat+by+neurotransmitters.">Modulation of Drosophila heartbeat by neurotransmitters.</a> J. Comp. Physiol. B. 167, 89-97 (1997).</li><li>Johnson, E., Ringo, J., Bray, N., Dowse, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+and+pharmacological+identification+of+ion+channels+central+to+the+Drosophila+cardiac+pacemaker.">Genetic and pharmacological identification of ion channels central to the Drosophila cardiac pacemaker.</a> J. Neurogenetics. 12, 1-24 (1998).</li><li>Johnson, E., Ringo, J., Dowse, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Native+and+heterologous+neuropeptides+are+cardioactive+in+Drosophila+melanogaster.">Native and heterologous neuropeptides are cardioactive in Drosophila melanogaster.</a> J. Insect Physiol. 1, 1229-1236 (2000).</li><li>Johnson, E., Sherry, T., Ringo, J., Dowse, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+the+cardiac+pacemaker+of+Drosophila:+cellular+mechanisms.">Modulation of the cardiac pacemaker of Drosophila: cellular mechanisms.</a> J. Comp. Physiol. B, Biochem., Systemic, Environmental Physiol. 172, 227-236 (2002).</li><li>Lewis, E. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+standard+food+medium.">A new standard food medium.</a> Drosophila Inform. Serv. 34, 117-117 (1960).</li><li>Miller, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+circulation+in+insects.">Control of circulation in insects.</a> General Pharmacol. 29, 23-38 (1997).</li><li>Molina, M. R., Cripps, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ostia,+the+inflow+tracts+of+the+Drosophila+heart,+develop+from+a+genetically+distinct+subset+of+cardial+cells.">Ostia, the inflow tracts of the Drosophila heart, develop from a genetically distinct subset of cardial cells.</a> Mechanisms of Development. 118, 51-59 (2001).</li><li>Muller, K. J., Nicholls, J. G., Stent, G. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Neurobiology of the Leech. , 254-254 (1981).</li><li>Nichols, R., Kaminski, S., Walling, E., Zornik, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulating+the+activity+of+a+cardioacceleratory+peptide.">Regulating the activity of a cardioacceleratory peptide.</a> Peptides. 20, 1153-1158 (1999).</li><li>Ocorr, K., Akasaka, T., Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Age-related+cardiac+disease+model+of+Drosophila.">Age-related cardiac disease model of Drosophila.</a> Mechanisms of Ageing and Development. 128, 112-116 (2007a).</li><li>Ocorr, K. A., Crawley, T., Gibson, G., Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+variation+for+cardiac+dysfunction+in+Drosophila.">Genetic variation for cardiac dysfunction in Drosophila.</a> PLoS ONE. 2, e601-e601 (2007).</li><li>Papaefthmiou, C., Theophilidis, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+in+vitro+method+for+recording+the+electrical+activity+of+the+isolated+heart+of+the+adult+Drosophila+melanogaster.">An in vitro method for recording the electrical activity of the isolated heart of the adult Drosophila melanogaster.</a> In Vitro Cellular &#38; Developmental Biol. Animal. 37, 445-449 (2001).</li><li>Peron, S., Zordan, M. A., Magnabosco, A., Reggiani, C., Megighian, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+action+potential+to+contraction:+Neural+control+and+excitation-contraction+coupling+in+larval+muscles+of+Drosophila.">From action potential to contraction: Neural control and excitation-contraction coupling in larval muscles of Drosophila.</a> Comp. Biochem.Physiol. A: Molecular &#38; Integrative Physiol. 154, 173-183 (2009).</li><li>Ponzielli, R., Astier, M., Chartier, A., Gallet, A., Therond, P., Semeriva, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+tube+patterning+in+Drosophila+requires+integration+of+axial+and+segmental+information+provided+by+the+Bithorax+Complex+genes+and+hedgehog+signaling.">Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling.</a> Develop. 129, 4509-4521 (2002).</li><li>Sl ma, K., Farkas, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heartbeat+patterns+during+the+postembryonic+development+of+Drosophila+melanogaster.">Heartbeat patterns during the postembryonic development of Drosophila melanogaster.</a> J. Insect Physiol. 51, 489-503 (2005).</li><li>St hmer, W., Roberts, W. S., Almers, W., Sakmann, B., Neher, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Single channel recordings. The loose patch clamp, 123-132 (1983).</li><li>Wessells, R. J., Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Screening+assays+for+heart+function+mutants+in+Drosophila.">Screening assays for heart function mutants in Drosophila.</a> Biotechniques. 37, 58-66 (2004).</li><li>Zornik, E., Paisley, K., Nichols, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+transmitters+and+a+peptide+modulate+Drosophila+heart+rate.">Neural transmitters and a peptide modulate Drosophila heart rate.</a> Peptides. 20, 45-51 (1999).</li></ol>

<ol><li> Strumenti di dissezione: Fine # 5 pinzette e forbici fine (tutti ottenuti da Strumenti Scienze Fine (USA), Inc., 373-G Vintage Park Drive, Foster City, CA 94404-1139) </li><li> Dissezione microscopio con funzione di zoom per la dissezione e la frequenza cardiaca di conteggio. Per la stimolazione focale del cuore questo è necessario pure. </li><li> Per le registrazioni extracellulari e intracellulari un microscopio composto con obiettivi in ​​posizione verticale (4 x e 20X) viene utilizzato. Standard amplificatore intracellulare e A / D a bordo per la registrazione in linea a un computer. I segnali elettrici vengono registrati in linea ad una interfaccia PowerLab/4s (ADInstruments, Australia). Noi utilizziamo software standard da ADInstruments nome o Campo Grafico. </li><li> Prodotti chimici: Tutti i prodotti chimici saline sono ottenuti da Sigma Chemical Company (St. Louis, MO). </li><li> Per le registrazioni intracellulari usiamo tubo capillare di vetro (catalogo # 30-31-0 da FHC, Brunswick, ME, 04011, USA) e per la registrazione macropatch focali e gli elettrodi stimolanti usiamo Kimax-51, Kimble Art Prodotti. N. 34502, ID 0.8-1.1mm, lunghezza 100mm. L&#39;elettrodo intracellulare dovrebbe avere una resistenza di 20 a 30 mOhm. L&#39;elettrodo macropatch è costruito rompendo la punta di un vetro dopo una punta fine è stata fatta da un elettrodo estrattore. La punta fuori rotta deve essere una rottura perpendicolare pulito circa 20μM di diametro. La punta è poi il calore lucidato a circa 10μM diametro interno per la registrazione focale extracellulare. Per l&#39;elettrodo stimolante per guidare il cuore che usiamo una punta finale di circa 20-50 micron di diametro. L&#39;albero dell&#39;elettrodo viene investito un elemento riscaldante per indurla a piegarsi di circa 45 gradi con una curva graduale. Questo produce un appartamento o lume elettrodo perpendicolare sul tubo cuore come l&#39;angolo con il micro-manipolatore produrrà circa altri 45 gradi rispetto alla preparazione. </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

Vi presentiamo vari metodi per registrare la funzione cardiaca nei larvale<em&gt; Drosophila</em&gt;. Gli approcci permettono la frequenza cardiaca di essere misurato in sfrenato e trattenuto larve intero. Per il controllo diretto dell&#39;ambiente intorno al cuore un altro approccio sfrutta le larve sezionato e la rimozione degli organi interni in modo da bagnare il cuore in composti desiderato. Il cuore esposto consente inoltre di potenziali di membrana da monitorare che può dare comprensione delle correnti ioniche generate dal miociti e di conduzione elettrica lungo il tubo cuore. Questi approcci hanno vantaggi e svantaggi per gli esperimenti futuri che sono discussi. La preparazione cuore larvale fornisce un modello aggiuntivo oltre ai<em&gt; Drosophila</em&gt; Scheletrico NMJ per indagare il ruolo della regolazione del calcio intracellulare sulla funzione cellulare. Conoscere meglio le correnti ioniche di fondo che danno forma al potenziali d&#39;azione in miociti in varie specie, si può sperare di ottenere un handle sul disfunzioni noto ionici associati a specifici geni responsabili di varie malattie nei mammiferi.

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Vi presentiamo vari modi per monitorare la funzione cardiaca in larva di<em&gt; Drosophila</em&gt; Per valutare le domande che fare con la funzione delle giunzioni gap, le mutazioni dei canali ionici, modulazione dell&#39;attività pacemaker e degli studi farmacologici.

Il monitoraggio delle funzioni cardiaca in larvali Drosophila melanogaster Per gli Studi fisiologici

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

monitoring-heart-function-larval-drosophila-melanogaster-for

Research

JoVE Journal

Biology

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

18.7K Views.  University of Kentucky, Lexington. Vi presentiamo vari modi per monitorare la funzione cardiaca in larva di Drosophila Per valutare le domande che fare con la funzione delle giunzioni gap, le mutazioni dei canali ionici, modulazione dell'attività pacemaker e degli studi farmacologici.