Un dispositivo a microfluidi con pattern Groove per studiare il comportamento cellulare

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12.3K Views

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13:50 min

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August 30th, 2007

DOI :

10.3791/270-v

August 30th, 2007

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Bong Geun Chung¹, Amir Manbachi¹, Ali Khademhosseini¹

¹Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

Riepilogo

Esplora Altri Video

Numero 7

biologia cellulare

ingegneria dei tessuti

microfluidica

l apoptosi

Capitoli in questo video

4:21

Assembly of microfluidic device

4:55

Cutting layers of microfluidic device

0:00

Creating channel inlet and outlet

1:27

Microfabrication of microfluidic device

6:10

Bonding the microfluidic device layers

9:09

Cell seeding

1:45

Preparation of PDMS mixture

2:42

Patterning PDMS molds

0:06

Introduction

3:36

Removing bubbles by vacuum

13:32

Time lapse of apoptosis

11:31

Application of reagents

9:08

Experiment: monitoring apoptosis

8:19

Coating channels with fibronectin

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