Microfabrication A. del dispositivo a microfluidi <ol><li> 4-pollici wafer Si è trattata con plasma di ossigeno reattivo (5 min a 30 W, Harrick scientifico, NY). </li><li> Photoresist negativo (SU-8 2015, MicroChem, MA) è spin-rivestito a 900 rpm per 1 minuto su un wafer di silicio. </li><li> Il wafer è morbido al forno a 95 ° C per 6 minuti su una piastra ed è esposto alla luce UV (200W) per 4 minuti attraverso un film maschera contenente microcanali. </li><li> Il wafer è post forno a 95 ° C per 6 minuti ed è stato sviluppato utilizzando SU-8 sviluppatore photoresist. </li><li> Il wafer photoresist fantasia contenente microcanali è posto in un piatto di Petri-. </li><li> Stampi poli (dimetilsiloxano) (PDMS) (Sylgard 184) sono fabbricati da elastomero di silicone di miscelazione e catalizzatore (10:1 ratio). </li><li> La miscela PDMS si versa nello stampo e maestro Si è posto su un essiccatore a vuoto per rimuovere le bolle. </li><li> PDMS è curata a 70 ° C per 1 ~ 2 ore. </li><li> Stampi PDMS sono poi staccate dallo stampo maestro Si. </li></ol> B. Montaggio del dispositivo <ol><li> 40 micron di spessore superiore canali fluidici e 40 micron di spessore inferiore canali microgrooved (25, 50, 75 e 100 μ m di larghezza) sono ottenuti da due diversi stampi Si maestro. </li><li> Canale di ingresso e uscita del dispositivo fluidici sono perforate. </li><li> Fluidico canali e canali microsolco sono irreversibilmente legati dal plasma di ossigeno reattivo (5 min a 30 W, Harrick scientifico, NY). </li><li> Matrice extracellulare (ECM) (ossia fibronectina) è rivestito all&#39;interno di dispositivo a microfluidi per 1 ora in incubatore (37 ° C). </li></ol> C. semina cellulare e sperimentale <ol><li> NIH-3T3 fibroblasti di topo sono coltivate in un pallone di coltura di tessuti utilizzando Dulbecco Modified Eagle Media (DMEM) contenente 10% siero fetale bovino (FBS). </li><li> Le cellule sono trypsinized e dissociato. </li><li> Cellule dissociate vengono caricati nei canali microsolco alla densità cellulare di 3 × 10 6 cellule / ml (Figura 1). <img src="/files/ftp_upload/old/270_2008_8_24_3/270_Figure-01.png" alt="Figura 1" width="191" height="300" /> Figura 1 </li><li> 2 media ml, 100 mm H 2 O 2, e il dosaggio apoptosi (20 microlitri annessina V e ioduro di propidio 40 microlitri, Invitrogen, CA) sono infuso in un canale utilizzando una pompa a siringa (1 ml / min). </li><li> Le cellule sono monitorate in tempo reale utilizzando un microscopio invertito (Nikon TE 2000). </li></ol>

<ol>
	<li>Chung, B. G., Park, J. W., Hu, J. S., Huang, C., Monuki, E. S., Jeon, N. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+hybrid+microfluidic-vacuum+device+for+interfacing+with+conventional+cell+culture+platform.">A hybrid microfluidic-vacuum device for interfacing with conventional cell culture platform.</a> BMC Biotechnol. 7, (2007).</li><li>Khademhosseini, A., Yeh, J., Eng, G., Karp, J., Kaji, H., Borenstein, J., Farokhzad, O. C., Langer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+docking+inside+microwells+within+reversibly+sealed+microfluidic+channels+for+fabricating+multiphenotype+cell+arrays.">Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays.</a> Lab Chip. 5, 1380-1386 (2005).</li><li>Whittemore, E. R., Loo, D. T., Watt, J. A., Cotman, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+detailed+analysis+of+hydrogen+peroxide-induced+cell+death+in+primary+neuronal+culture.">A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture.</a> Neuroscience. 67, 921-932 (1995).</li></ol>

Le cellule sono state immobilizzate e fantasia all&#39;interno di substrati microgrooved in un dispositivo a microfluidi. Il processo di apoptosi delle cellule esposte al perossido di idrogeno è stata osservata in tempo reale e analizzato utilizzando annessina V e ioduro di propidio. Quindi, questo dispositivo a microfluidi contenenti canali microsolco potrebbe essere utile per high-throughput screening di stupefacenti.

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>PDMS</td>
<td>Reagent</td>
<td>K.R. Anderson Co. Inc. </td>
<td>2065622 </td>
<td>Poly(dimethylsiloxane), Dow Corning Sylgard 184 (8.6 lb)</td>
</tr>
<tr>
<td>DMEM</td>
<td>medium</td>
<td>Invitrogen</td>
<td>11965 </td>
<td>Dulbecco&#x2019;s Modified Eagle&#x2019;s Media </td>
</tr>
<tr>
<td>FBS</td>
<td>serum</td>
<td>Invitrogen</td>
<td>10082-147 </td>
<td>Fetal Bovine Serum
</td>
</tr>
<tr>
<td>Hydrogen peroxide</td>
<td>Reagent</td>
<td>Sigma Aldrich</td>
<td> H1009 </td>
<td>&nbsp;</td>
<tr>
<td>Apoptosis assay</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>V13242 </td>
<td>Annexin A, propidium iodide</td>
</tr>
<tr>
<td>Negative photoresist </td>
<td>&nbsp;</td>
<td>Microchem</td>
<td>SU-8 2015</td>
<td>&nbsp;</td>
<tr>
<td>Si wafer </td>
<td>Tool</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4 inch silicone wafer</td>
</tr>
<tr>
<td>Reactive oxygen plasma </td>
<td>Reagent</td>
<td>Harrick Scientific</td>
<td>&nbsp;</td>
<td>treat wafer 5 min at 30W</td>
</tr>
<tr>
<td>inverted microscope </td>
<td>Tool</td>
<td>Nikon </td>
<td>TE 2000</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>
			<div>
					</div>

a microfluidic device with groove patterns for studying cellular behavior

Descriviamo un dispositivo a microfluidi con modelli microgrooved per studiare il comportamento cellulare. Questa piattaforma microfluidica consiste in un canale superiore fluidico e un substrato di fondo microgrooved. Per fabbricare i canali microgrooved, un top poli (dimetilsiloxano) (PDMS) muffa contenente l&#39;impressione dei canali microfluidica era allineato e legato ad un substrato microgrooved. Utilizzando questo dispositivo, le cellule di fibroblasti del mouse sono stati immobilizzati e fantasia all&#39;interno di substrati microgrooved (25, 50, 75 e 100 micron di larghezza). Per studiare l&#39;apoptosi in un dispositivo a microfluidi, supporti contenenti perossido di idrogeno, annessina V e ioduro di propidio è stato perfuso nel canale fluidico per 2 ore. Abbiamo trovato che le cellule esposte a stress ossidativo è diventato apoptotico. Queste cellule apoptotiche sono state confermate da annessina V che si lega alla fosfatidilserina al foglietto esterno della membrana plasmatica durante il processo di apoptosi. L&#39;utilizzo di questo dispositivo a microfluidi con motivi microgrooved, il processo di apoptosi è stata osservata in tempo reale e analizzati utilizzando un microscopio invertito che contiene una camera di incubazione (37 ° C, 5% CO 2). Pertanto, questo dispositivo a microfluidi incorporato con substrati microgrooved potrebbe essere utile per studiare il comportamento cellulare e l&#39;esecuzione di high-throughput screening di stupefacenti.

Watch this Scientific Journal Video about A Microfluidic Device with Groove Patterns for Studying Cellular Behavior at JoVE.com

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Abbiamo descritto un protocollo per la fabbricazione di dispositivi microfluidici che può consentire la cattura delle cellule e della cultura. In questo approccio microstrutture modellata come scanalature all&#39;interno dei canali microfluidica sono utilizzati per creare le regioni a basso sforzo di taglio all&#39;interno del quale cellula possono attraccare.

Un dispositivo a microfluidi con pattern Groove per studiare il comportamento cellulare

We describe a microfluidic device with microgrooved patterns for studying cellular behavior.  This microfluidic platform consists of a top fluidic channel ...

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Research

JoVE Journal

Biology

12.3K Views.  Brigham and Women's Hospital. Abbiamo descritto un protocollo per la fabbricazione di dispositivi microfluidici che può consentire la cattura delle cellule e della cultura. In questo approccio microstrutture modellata come scanalature all'interno dei canali microfluidica sono utilizzati per creare le regioni a basso sforzo di taglio all'interno del quale cellula possono attraccare.

Video: A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

In this video, we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to fabricate a microfluidic device for culturing neurons. Previously, a silicon wafer was patterned with the design for the neuron microfluidic device using SU-8 and photolithography to create a master mold, or what we simply refer to as a "master". Next, we pour the silicon polymer PDMS on top of the master which is then cured by heating the PDMS to 80&deg;C for 1 hour. The PDMS forms a negative mold of the device. The PDMS is then carefully cut and lifted away from the master. Holes are punched where the reservoirs will be and the excess PDMS trimmed away from the device. Nitrogen is used to blow away any excess debris from the device. At this point the devices are now ready for use and can either bonded to corning No. 1 cover glass with a plasma sterilizer/cleaner or can be reversibly bound to the cover glass by simply placing the device on top of the cover glass. The reversible bonding of the device to glass is covered in a separate video and requires first that the device be sterilized either with 70% ethanol or by autoclaving. Plasma treating sterilizes the devices so no further treatment is necessary. It is, however, important, when plasma-treating the devices, to add liquid to the devices within 10 minutes of the plasma treatment while the surfaces are still hydrophilic. Waiting longer than 10 minutes to add liquid to the device makes it difficult for the liquid to enter the device. The neuron devices are typically plasma-bound to cover glass and 0.5 mg/ml poly-L-lysine (PLL) in pH 8.5 borate buffer is immediately added to the device. After a minimum of 3 hours incubating with PLL, the devices are washed with dH2O water a minimum of 3 times with at least 15 minutes between each wash. Next, the water is removed and fresh media is added to the device. At this point the device is ready for use. It is important to remember at this point to never remove all the media from the device. Always leave media in the main channel.

In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.

Fabbricazione di un dispositivo a microfluidi per la compartimentazione dei Neuron Soma e assoni

In this video, we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to fabricate a microfluidic device for ...

Ricerca

Biologia

A Gradient-generating Microfluidic Device for Cell Biology

 The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described.  A microfluidic platform is an enabling experimental tool, because it can precisely manipulate fluid flows, enable high-throughput experiments, and generate stable soluble concentration gradients.  Compared to conventional gradient generators, poly(dimethylsiloxane) (PDMS)-based  microfluidic devices can generate stable concentration gradients of growth factors with well-defined profiles.  Here, we developed simple gradient-generating microfluidic devices with three separate inlets.  Three microchannels combined into one microchannel to generate concentration gradients.  The stability and shape of growth factor gradients were confirmed by fluorescein isothyiocyanate (FITC)-dextran with a molecular weight similar to epidermal growth factor (EGF).  Using this microfluidic device, we demonstrated that fibroblasts exposed to concentration gradients of EGF migrated toward higher concentrations.  The directional orientation of cell migration and motility of migrating cells were quantitatively assessed by cell tracking analysis.  Thus, this gradient-generating microfluidic device might be useful for studying and analyzing the behavior of migrating cells.

We describe a protocol for the microfabrication of the gradient-generating microfluidic device that can generate spatial and temporal gradients in well-defined microenvironment. In this approach, the gradient-generating microfluidic device can be used to study directed cell migration, embryogenesis, wound healing, and cancer metastasis.

Un gradiente di generazione del dispositivo Microfluidic di Biologia Cellulare

 The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described.  A microfluidic platform is an ...

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously dissected and prepared. The E18 cortex is, upon dissection, immediately dissociated into individual neurons. It is possible to store E18 cortex in Hibernate E buffer containing B27 at 4&deg;C for up to a week before the dissociation is performed. However, there will be a drop in cell viability. Typically we obtain our E18 Cortex fresh. It is transported to the lab in ice cold Calcium free Magnesium free dissection buffer (CMFM). Upon arrival, trypsin is added to the cortex to a final concentration of 0.125%. The cortex is then incubated at 37&deg;C for 8 minutes. DMEM containing 10% FBS is added to the cortex to stop the reaction. The cortex is then centrifuged at 2500 rpm for 2 minutes. The supernatant is removed and 2 ml of Neural Basal Media (NBM) containing 2% B27 (vol/vol) and 0.25% Glutamax (vol/vol) is added to the cortex which is then re-suspended by pipetting up and down. Next, the cortex is triturated with previously fire polished glass pipettes, each with a successive smaller opening. After triturating, the cortex is once again centrifuged at 2500 rpm for 2 minutes. The supernatant is then removed and the cortex pellet re-suspended with 2 ml of NBM containing B27 and Glutamax. The cell suspension is then passed through a 40 um nylon cell strainer. Next the cells are counted. The neurons are now ready for loading into the neuron microfluidic device.

In this video we demonstrate the preparation of E18 Cortical Rat Neurons.

Preparazione E18 neuroni corticali di ratto per compartimentazione in un dispositivo a microfluidi

In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously ...

Cell Capture Using a Microfluidic Device

Cattura cellula Utilizzando un dispositivo a microfluidi

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is largely due to the difficulty with which one can create a cell-compatible and steady microenvironment using conventional microfabrication techniques and materials. We address this problem via the introduction of a novel microfabrication material, agarose gel, as the base material for the microfluidic device. Agarose gel is highly malleable, and permeable to gas and nutrients necessary for cell survival, and thus an ideal material for cell-based assays. We have shown previously that agarose gel based devices have been successful in studying bacterial and neutrophil cell migration2. In this report, three parallel microfluidic channels are patterned in an agarose gel membrane of about 1mm thickness. Constant flows with media/buffer are maintained in the two side channels using a peristaltic pump. Cells are maintained in the center channel for observation. Since the nutrients and chemicals in the side channels are constantly diffusing from the side to center channel, the chemical environment of the center channel is easily controlled via the flow along the side channels. Using this device, we demonstrate that the movement of neural stem cells can be monitored optically with ease under various chemical conditions, and the experimental results show that the over expression of epidermal growth factor receptors (EGFR) enhances the motility of neural stem cells. Motility of neural stem cells is an important biomarker for assessing cells aggressiveness, thus tumorigenic factor3. Deciphering the mechanism underlying NSC motility will yield insight into both disorders of neural development and into brain cancer stem cell invasion.

We demonstrate that the over expression of epidermal growth factor receptors (EGFR) enhances the motility of neural stem cells(NSCs) using a novel agarose gel based microfluidic device. This technology can be readily adaptable to other mammalian cell systems where cell sources are scarce, such as human neural stem cells, and the turn around time is critical.

Valutare motilità delle cellule staminali neurali L&#39;utilizzo di un gel a base di Agarose dispositivo a microfluidi

While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is ...

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

This protocol describes the use of the Drosophila melanogaster syncytial embryo for studying mitosis1. Drosophila has useful genetics with a sequenced genome, and it can be easily maintained and manipulated. Many mitotic mutants exist, and transgenic flies expressing functional fluorescently (e.g. GFP) - tagged mitotic proteins have been and are being generated. Targeted gene expression is possible using the GAL4/UAS system2. 

The Drosophila early embryo carries out multiple mitoses very rapidly (cell cycle duration, &asymp;10 min). It is well suited for imaging mitosis, because during cycles 10-13, nuclei divide rapidly and synchronously without intervening cytokinesis at the surface of the embryo in a single monolayer just underneath the cortex. These rapidly dividing nuclei probably use the same mitotic machinery as other cells, but they are optimized for speed; the checkpoint is generally believed to not be stringent, allowing the study of mitotic proteins whose absence would cause cell cycle arrest in cells with a strong checkpoint. Embryos expressing GFP labeled proteins or microinjected with fluorescently labeled proteins can be easily imaged to follow live dynamics (Fig. 1). In addition, embryos can be microinjected with function-blocking antibodies or inhibitors of specific proteins to study the effect of the loss or perturbation of their function3. These reagents can diffuse throughout the embryo, reaching many spindles to produce a gradient of concentration of inhibitor, which in turn results in a gradient of defects comparable to an allelic series of mutants. Ideally, if the target protein is fluorescently labeled, the gradient of inhibition can be directly visualized4. It is assumed that the strongest phenotype is comparable to the null phenotype, although it is hard to formally exclude the possibility that the antibodies may have dominant effects in rare instances, so rigorous controls and cautious interpretation must be applied. Further away from the injection site, protein function is only partially lost allowing other functions of the target protein to become evident.

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

This protocol describes the use of microinjection and high resolution imaging in the Drosophila melanogaster syncytial embryo to study mitosis.

Tecniche di microiniezione per Studiare Mitosi in Drosophila melanogaster Embryo sinciziale

This protocol describes the use of the Drosophila melanogaster syncytial embryo  for studying mitosis1.  Drosophila has useful genetics with a sequenced ...

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes. The device consists of three layers: first layer contains an array of microscale wells (500 &mu;m diameter and 150 &mu;m depth) that help to immobilize the islets while exposed to flow and maximize the exposed surface area of the islets; the second layer contains a circular perifusion chamber (3 mm deep, 7 mm diameter); and the third layer contains an inlet-mixing channel that fans out before injection into the perifusion chamber (2 mm in width, 19 mm in length, and 500 &mu;m in height) for optimizing the mixing efficiency prior to entering the perifusion chamber. The creation of various glucose gradients including a linear, bell shape, and square shapes also can be created in the microfluidic perifusion network and is demonstrated.

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes.

Un multi-parametrica Perifusion Sistema isolotto all&#39;interno di un dispositivo Perifusion Microfluidic

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence ...

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media

Microbial growth and transport in porous media have important implications for the quality of groundwater and surface water, the recycling of nutrients in the environment, as well as directly for the transmission of pathogens to drinking water supplies. Natural porous media is composed of an intricate physical topology, varied surface chemistries, dynamic gradients of nutrients and electron acceptors, and a patchy distribution of microbes. These features vary substantially over a length scale of microns, making the results of macro-scale investigations of microbial transport difficult to interpret, and the validation of mechanistic models challenging. Here we demonstrate how simple microfluidic devices can be used to visualize microbial interactions with micro-structured habitats, to identify key processes influencing the observed phenomena, and to systematically validate predictive models. Simple, easy-to-use flow cells were constructed out of the transparent, biocompatible and oxygen-permeable material poly(dimethyl siloxane). Standard methods of photolithography were used to make micro-structured masters, and replica molding was used to cast micro-structured flow cells from the masters. The physical design of the flow cell chamber is adaptable to the experimental requirements: microchannels can vary from simple linear connections to complex topologies with feature sizes as small as 2 &mu;m. Our modular EcoChip flow cell array features dozens of identical chambers and flow control by a gravity-driven flow module. We demonstrate that through use of EcoChip devices, physical structures and pressure heads can be held constant or varied systematically while the influence of surface chemistry, fluid properties, or the characteristics of the microbial population is investigated. Through transport experiments using a non-pathogenic, green fluorescent protein-expressing Vibrio bacterial strain, we illustrate the importance of habitat structure, flow conditions, and inoculums size on fundamental transport phenomena, and with real-time particle-scale observations, demonstrate that microfluidics offer a compelling view of a hidden world.

Microfluidic devices can be used to visualize complex natural processes in real time and at the appropriate physical scales. We have developed a simple microfluidic device that mimics key features of natural porous media for studying growth and transport of bacteria in the subsurface.

Finestra su un micromondo: Sistemi Microfluidic semplice per studiare il trasporto microbica in mezzi porosi

Microbial growth and transport in porous media have important implications for the quality of groundwater and surface water, the recycling of nutrients in ...

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the concentration of specific chemoeffectors by comparing the current concentration to the concentration detected a few seconds earlier. This comparison determines the net direction of movement. Although multiple, competing gradients often coexist in nature, conventional approaches for investigating bacterial chemotaxis are suboptimal for quantifying migration in response to concentration gradients of attractants and repellents. Here, we describe the development of a microfluidic chemotaxis model for presenting precise and stable concentration gradients of chemoeffectors to bacteria and quantitatively investigating their response to the applied gradient. The device is versatile in that concentration gradients of any desired absolute concentration and gradient strength can be easily generated by diffusive mixing. The device is demonstrated using the response of Escherichia coli RP437 to gradients of amino acids and nickel ions.

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

Un dispositivo a microfluidi per quantificare chemiotassi batterica in gradienti di concentrazione stabile

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

Non-coding genomic regions in complex eukaryotes, including intergenic areas, introns, and untranslated segments of exons, are profoundly non-random in their nucleotide composition and consist of a complex mosaic of sequence patterns. These patterns include so-called Mid-Range Inhomogeneity (MRI) regions -- sequences 30-10000 nucleotides in length that are enriched by a particular base or combination of bases (e.g. (G+T)-rich, purine-rich, etc.). MRI regions are associated with unusual (non-B-form) DNA structures that are often involved in regulation of gene expression, recombination, and other genetic processes (Fedorova &amp; Fedorov 2010). The existence of a strong fixation bias within MRI regions against mutations that tend to reduce their sequence inhomogeneity additionally supports the functionality and importance of these genomic sequences (Prakash et al. 2009).
Here we demonstrate a freely available Internet resource -- the Genomic MRI program package -- designed for computational analysis of genomic sequences in order to find and characterize various MRI patterns within them (Bechtel et al. 2008). This package also allows generation of randomized sequences with various properties and level of correspondence to the natural input DNA sequences. The main goal of this resource is to facilitate examination of vast regions of non-coding DNA that are still scarcely investigated and await thorough exploration and recognition.

We present a public computational web site for the analysis of genomic sequences. It detects DNA sequence patterns with various non-random nucleotide compositions. This resource also generates randomized sequences with diverse levels of complexity.

Genomica risonanza magnetica - una risorsa pubblica per lo Studio Patterns sequenza all&#39;interno del DNA genomico

Non-coding genomic regions in complex eukaryotes, including intergenic areas, introns, and untranslated segments of exons, are profoundly non-random in ...